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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CLINT1 Polyclonal antibody (10470-1-AP)

    WB analysis of HeLa using 10470-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10470-1-AP (CLINT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CLINT1 Polyclonal antibody (10470-1-AP)

    WB analysis using 10470-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10470-1-AP (CLINT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CLINT1 Polyclonal antibody (10470-1-AP)

    WB analysis of HEK-293 using 10470-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10470-1-AP (CLINT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using CLINT1 Polyclonal antibody (10470-1-AP)

    IP experiment of HeLa using 10470-1-AP

    IP result of anti-CLINT1 (IP:10470-1-AP, 4ug; Detection:10470-1-AP 1:1000) with HeLa cells lysate 2800ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CLINT1 Polyclonal antibody (10470-1-AP)

    IHC staining of human breast cancer using 10470-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10470-1-AP (CLINT1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CLINT1 Polyclonal antibody (10470-1-AP)

    IHC staining of human breast cancer using 10470-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10470-1-AP (CLINT1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CLINT1 Polyclonal antibody (10470-1-AP)

    IF Staining of HepG2 using 10470-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CLINT1 antibody (10470-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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CLINT1 Polyclonal antibody

CLINT1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 10470-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

EPN4, Enthoprotin, ENTH, Clint, Clathrin-interacting protein localized in the trans-Golgi region

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells, HeLa cells, Jurkat cells, mouse brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

10470-1-AP targets CLINT1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CLINT1 fusion protein Ag0754 相同性解析による交差性が予測される生物種
Full Name clathrin interactor 1
Calculated molecular weight 68 kDa
Observed molecular weight 68 kDa, 80 kDa
GenBank accession numberBC004467
Gene Symbol CLINT1
Gene ID (NCBI) 9685
RRIDAB_2276405
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14677
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Clathrin interactor 1 (CLINT1), also known as enthoprotin or epsin-4, belongs to the epsin family of endocytic adapter proteins. CLINT1 interacts with clathrin, the adapter protein AP-1 and phosphoinositides. It may have a role in the formation of clathrin coated vesicles and trafficking between the trans-Golgi network and endosomes. Mutations in the gene of CLINT1 are associated with a susceptibility to schizophrenia and psychotic disorders. Calculated molecular weight of CLINT1 is 68 kDa. The migration of endogenous CLINT1 at 80 kDa has also been reported (PMID: 12213833; 15811338).

Protocols

Product Specific Protocols
WB protocol for CLINT1 antibody 10470-1-APDownload protocol
IHC protocol for CLINT1 antibody 10470-1-APDownload protocol
IF protocol for CLINT1 antibody 10470-1-APDownload protocol
IP protocol for CLINT1 antibody 10470-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

BioE3 identifies specific substrates of ubiquitin E3 ligases

Authors - Orhi Barroso-Gomila
View Article
WB

bioRxiv

PKA Activity-Driven Modulation of Bidirectional Long-Distance transport of Lysosomal vesicles During Synapse Maintenance

Authors - Kerriann K Badal
View Article