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  • Featured Product
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CLIP-115/CLIP2 Monoclonal antibody

CLIP-115/CLIP2 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, rabbit

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2E6B6

Cat no : 67776-1-Ig

Synonyms

CLIP, CLIP 115, CLIP-115/CLIP2, CLIP2, CYLN2, Cytoplasmic linker protein 115, Cytoplasmic linker protein 2, KIAA0291, WBSCR3, WBSCR4, WSCR3, WSCR4

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse cerebellum tissue using CLIP-115/CLIP2 Monoclonal antibody (67776-1-Ig)

    WB analysis of mouse cerebellum using 67776-1-Ig

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 67776-1-Ig (CLIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using CLIP-115/CLIP2 Monoclonal antibody (67776-1-Ig)

    WB analysis of A549 using 67776-1-Ig

    WB result of CLIP-115/CLIP2 antibody (67776-1-Ig; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-CLIP-115/CLIP2 transfected A549 cells.

  • Western Blot (WB) analysis of rabbit brain tissue using CLIP-115/CLIP2 Monoclonal antibody (67776-1-Ig)

    WB analysis of rabbit brain using 67776-1-Ig

    rabbit brain tissue were subjected to SDS PAGE followed by western blot with 67776-1-Ig (CLIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using CLIP-115/CLIP2 Monoclonal antibody (67776-1-Ig)

    WB analysis of pig brain using 67776-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67776-1-Ig (CLIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using CLIP-115/CLIP2 Monoclonal antibody (67776-1-Ig)

    WB analysis of rat brain using 67776-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 67776-1-Ig (CLIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using CLIP-115/CLIP2 Monoclonal antibody (67776-1-Ig)

    WB analysis of mouse brain using 67776-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67776-1-Ig (CLIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig cerebellum tissue using CLIP-115/CLIP2 Monoclonal antibody (67776-1-Ig)

    WB analysis of pig cerebellum using 67776-1-Ig

    pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 67776-1-Ig (CLIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat cerebellum tissue using CLIP-115/CLIP2 Monoclonal antibody (67776-1-Ig)

    WB analysis of rat cerebellum using 67776-1-Ig

    rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 67776-1-Ig (CLIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CLIP-115/CLIP2 Monoclonal antibody (67776-1-Ig)

    IF Staining of A549 using 67776-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CLIP-115/CLIP2 antibody (67776-1-Ig, Clone: 2E6B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inmouse cerebellum tissue, rabbit brain tissue, pig brain tissue, rat brain tissue, mouse brain tissue, pig cerebellum tissue, rat cerebellum tissue, A549 cells
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67776-1-Ig targets CLIP-115/CLIP2 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Reactivity Human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CLIP-115/CLIP2 fusion protein Ag18902 相同性解析による交差性が予測される生物種
Full Name CAP-GLY domain containing linker protein 2
Calculated molecular weight 1011 aa, 112 kDa
Observed molecular weight120 kDa
GenBank accession numberBC150332
Gene symbol CLIP2
Gene ID (NCBI) 7461
RRIDAB_2918541
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CILP-2/CLIP-115 is a cytoplasmic linker protein (CLIP) that links membranous organelles to microtubules. CLIP-115 is enriched in the axonal growth cone and it enables neuronal polarization by controlling the stabilization of microtubules. Mutation of CLIP-115 has been associated with Williams-Beuren syndrome.

Protocols

Product Specific Protocols
WB protocol for CLIP-115/CLIP2 antibody 67776-1-IgDownload protocol
IF protocol for CLIP-115/CLIP2 antibody 67776-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols