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  • Featured Product
  • KD/KO Validated

CLIP-115/CLIP2 Polyclonal antibody

CLIP-115/CLIP2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Cat no : 25088-1-AP

Synonyms

CLIP, CLIP 115, CLIP-115/CLIP2, CLIP2, CYLN2, Cytoplasmic linker protein 115, Cytoplasmic linker protein 2, KIAA0291, WBSCR3, WBSCR4, WSCR3, WSCR4

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using CLIP-115/CLIP2 Polyclonal antibody (25088-1-AP)

    WB analysis of A549 using 25088-1-AP

    WB result of CLIP-115/CLIP2 antibody (25088-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-CLIP-115/CLIP2 transfected A549 cells.

  • Western Blot (WB) analysis of various lysates using CLIP-115/CLIP2 Polyclonal antibody (25088-1-AP)

    WB analysis using 25088-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25088-1-AP (CLIP-115/CLIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using CLIP-115/CLIP2 Polyclonal antibody (25088-1-AP)

    WB analysis of mouse brain using 25088-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25088-1-AP (CLIP-115/CYLN2 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using CLIP-115/CLIP2 Polyclonal antibody (25088-1-AP)

    WB analysis of rat brain using 25088-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 25088-1-AP (CLIP-115/CYLN2 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using CLIP-115/CLIP2 Polyclonal antibody (25088-1-AP)

    IP experiment of mouse brain using 25088-1-AP

    IP result of anti-CLIP-115/CLIP2 (IP:25088-1-AP, 4ug; Detection:25088-1-AP 1:15000) with mouse brain tissue lysate 1080 ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CLIP-115/CLIP2 Polyclonal antibody (25088-1-AP)

    IHC staining of mouse brain using 25088-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25088-1-AP (CLIP-115/CLIP2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inRAW 264.7 cells, A549 cells, rat brain tissue, mouse brain tissue, mouse cerebellum tissue, rat cerebellum tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

25088-1-AP targets CLIP-115/CLIP2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CLIP-115/CLIP2 fusion protein Ag18692 相同性解析による交差性が予測される生物種
Full Name CAP-GLY domain containing linker protein 2
Calculated molecular weight 1011 aa, 112 kDa
Observed molecular weight 110-120 kDa
GenBank accession numberBC150332
Gene symbol CLIP2
Gene ID (NCBI) 7461
RRIDAB_2879890
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CILP-2/CLIP-115 is a cytoplasmic linker protein (CLIP) that links membranous organelles to microtubules. CLIP-115 is enriched in the axonal growth cone and it enables neuronal polarization by controlling the stabilization of microtubules. Mutation of CLIP-115 has been associated with Williams-Beuren syndrome.

Protocols

Product Specific Protocols
WB protocol for CLIP-115/CLIP2 antibody 25088-1-APDownload protocol
IHC protocol for CLIP-115/CLIP2 antibody 25088-1-APDownload protocol
IP protocol for CLIP-115/CLIP2 antibody 25088-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Curr Biol

Stretch triggers microtubule stabilization and MARCKS-dependent membrane incorporation in the shaft of embryonic axons

Authors - Sara C Sousa
View Article