CLIP1 Polyclonal antibody

Name: CLIP1 Polyclonal antibody
Brand: Proteintech
SKU: 23839-1-AP

CLIP1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 23839-1-AP

Synonyms

RSN, Reed-Sternberg intermediate filament-associated protein, Cytoplasmic linker protein 170 alpha-2, CYLN1, CLIP-170

Validation Data Gallery

Filter:

AllWBIPIF/ICC

WB analysis using 23839-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 23839-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of U-937 using 23839-1-AP
U-937 cells were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 23839-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 23839-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of rat kidney using 23839-1-AP
rat kidney tissue were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 23839-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 23839-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 23839-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 23839-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 23839-1-AP
IP result of anti-CLIP1 (IP:23839-1-AP, 4ug; Detection:23839-1-AP 1:2000) with HeLa cells lysate 2400ug.
IF Staining of HeLa using 23839-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 23839-1-AP (CLIP1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 23839-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, HEK-293 cells, MCF-7 cells, mouse kidney tissue, rat kidney tissue, U-937 cells
Positive IP detected in	HeLa cells
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
IF	See 1 publications below

Product Information

23839-1-AP targets CLIP1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CLIP1 fusion protein Ag20811 相同性解析による交差性が予測される生物種
Full Name	CAP-GLY domain containing linker protein 1
Calculated molecular weight	1438 aa, 162 kDa
Observed molecular weight	160-170 kDa, 135 kDa
GenBank accession number	BC126305
Gene symbol	CLIP1
Gene ID (NCBI)	6249
RRID	AB_2879334
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen Affinity purified
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for CLIP1 antibody 23839-1-AP	Download protocol
IF protocol for CLIP1 antibody 23839-1-AP	Download protocol
IP protocol for CLIP1 antibody 23839-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Front Cell Dev Biol TMPRSS12 Functions in Meiosis and Spermiogenesis and Is Required for Male Fertility in Mice. Authors - Jingjing Zhang View Article
human	WB	Cell Death Dis Tension of plus-end tracking protein Clip170 confers directionality and aggressiveness during breast cancer migration Authors - Yunfeng Hu View Article
human	IF	Science The mechanism of acentrosomal spindle assembly in human oocytes Authors - Tianyu Wu View Article
human	WB	Ecotoxicol Environ Saf Exosomal circCLIP1 regulates PM2.5-induced airway obstruction via targeting SEPT10 in vitro Authors - Huanhuan Zhu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CLIP1 Polyclonal antibody

CLIP1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 23839-1-AP

WB analysis of MCF-7 using 23839-1-AP

WB analysis of U-937 using 23839-1-AP

WB analysis of HEK-293 using 23839-1-AP

WB analysis of HEK-293 using 23839-1-AP

WB analysis of rat kidney using 23839-1-AP

WB analysis of mouse kidney using 23839-1-AP

WB analysis of HeLa using 23839-1-AP

WB analysis of HEK-293 using 23839-1-AP

WB analysis of MCF-7 using 23839-1-AP

IP experiment of HeLa using 23839-1-AP

IF Staining of HeLa using 23839-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

TMPRSS12 Functions in Meiosis and Spermiogenesis and Is Required for Male Fertility in Mice.

Tension of plus-end tracking protein Clip170 confers directionality and aggressiveness during breast cancer migration

The mechanism of acentrosomal spindle assembly in human oocytes

Exosomal circCLIP1 regulates PM2.5-induced airway obstruction via targeting SEPT10 in vitro