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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CLN6 Polyclonal antibody

CLN6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 20315-1-AP

Synonyms

CLN6, HsT18960, nclf, Protein CLN6

Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using CLN6 Polyclonal antibody (20315-1-AP)

    WB analysis of SH-SY5Y using 20315-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20315-1-AP (CLN6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CLN6 Polyclonal antibody (20315-1-AP)

    WB analysis of HEK-293 using 20315-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20315-1-AP (CLN6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using CLN6 Polyclonal antibody (20315-1-AP)

    WB analysis of SH-SY5Y using 20315-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20315-1-AP (CLN6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using CLN6 Polyclonal antibody (20315-1-AP)

    IP experiment of HEK-293 using 20315-1-AP

    IP result of anti-CLN6 (IP:20315-1-AP, 4ug; Detection:20315-1-AP 1:300) with HEK-293 cells lysate 2560ug.

  • Immunohistochemistry (IHC) staining of human colon tissue using CLN6 Polyclonal antibody (20315-1-AP)

    IHC staining of human colon using 20315-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20315-1-AP (CLN6 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using CLN6 Polyclonal antibody (20315-1-AP)

    IHC staining of human colon using 20315-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20315-1-AP (CLN6 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using CLN6 Polyclonal antibody (20315-1-AP)

    IHC staining of human small intestine using 20315-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20315-1-AP (CLN6 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using CLN6 Polyclonal antibody (20315-1-AP)

    IHC staining of human small intestine using 20315-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20315-1-AP (CLN6 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CLN6 Polyclonal antibody (20315-1-AP)

    IF Staining of HEK-293 using 20315-1-AP

    Immunofluorescent analysis of HEK-293 cells using 20315-1-AP (CLN6 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inSH-SY5Y cells, HEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 20315-1-AP is CLN6 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CLN6 fusion protein Ag14159 相同性解析による交差性が予測される生物種
Full Name ceroid-lipofuscinosis, neuronal 6, late infantile, variant
Calculated molecular weight 311 aa, 36 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC010849
Gene symbol CLN6
Gene ID (NCBI) 54982
RRIDAB_2878671
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CLN6 antibody 20315-1-APDownload protocol
IHC protocol for CLN6 antibody 20315-1-APDownload protocol
IF protocol for CLN6 antibody 20315-1-APDownload protocol
IP protocol for CLN6 antibody 20315-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Genet Metab

Novel mutations in CLN6 cause late-infantile neuronal ceroid lipofuscinosis without visual impairment in two unrelated patients.

Authors - Joseph J Chin
View Article