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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CLOCK Polyclonal antibody, PBS Only

CLOCK Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 30680-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Immunohistochemistry (IHC) staining of mouse liver tissue using CLOCK Polyclonal antibody (30680-1-AP)

    IHC staining of mouse liver using 30680-1-AP (same clone as 30680-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30680-1-AP (CLOCK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30680-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CLOCK Polyclonal antibody (30680-1-AP)

    IHC staining of mouse liver using 30680-1-AP (same clone as 30680-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30680-1-AP (CLOCK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30680-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CLOCK Polyclonal antibody (30680-1-AP)

    IF Staining of HeLa using 30680-1-AP (same clone as 30680-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CLOCK antibody (30680-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 30680-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30680-1-PBS targets CLOCK in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CLOCK fusion protein Ag33370 相同性解析による交差性が予測される生物種
Full Name clock homolog (mouse)
Calculated molecular weight 846 aa, 95 kDa
GenBank accession numberBC041878
Gene symbol CLOCK
Gene ID (NCBI) 9575
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Circadian locomoter output cycles protein kaput (CLOCK), also named as BHLHE8 or KIAA0334, is a 846 amino acid protein, which contains one bHLH domain, one PAC domain, and two PAS domains. CLOCK localizes in the nucleus and cytoplasm. CLOCK) is expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. ARNTL/2-CLOCK heterodimers activate E-box element (5'-CACGTG-3') transcription of a number of proteins of the circadian clock, such as PER1 and PER2. western blot. Our result is similar to the result of reseach paper (PMID: 30683868).