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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CLOCK Recombinant antibody, PBS Only

CLOCK Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1I23

Cat no : 82829-1-PBS

Synonyms

bHLHe8, CLOCK, clock homolog (mouse), hCLOCK, KAT13D, KIAA0334

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CLOCK Recombinant antibody (82829-1-RR)

    WB analysis using 82829-1-RR (same clone as 82829-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82829-1-RR (CLOCK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82829-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CLOCK Recombinant antibody (82829-1-RR)

    WB analysis using 82829-1-RR (same clone as 82829-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82829-1-RR (CLOCK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82829-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using CLOCK Recombinant antibody (82829-1-RR)

    WB analysis of HeLa using 82829-1-RR (same clone as 82829-1-PBS)

    WB result of CLOCK antibody (82829-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CLOCK transfected HeLa cells. This data was developed using the same antibody clone with 82829-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CLOCK Recombinant antibody (82829-1-RR)

    IHC staining of mouse liver using 82829-1-RR (same clone as 82829-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 82829-1-RR (CLOCK antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82829-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CLOCK Recombinant antibody (82829-1-RR)

    IHC staining of mouse liver using 82829-1-RR (same clone as 82829-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 82829-1-RR (CLOCK antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82829-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82829-1-PBS targets CLOCK in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CLOCK fusion protein Ag30011 相同性解析による交差性が予測される生物種
Full Name clock homolog (mouse)
Calculated molecular weight 846 aa, 95 kDa
Observed molecular weight110 kDa
GenBank accession numberBC041878
Gene symbol CLOCK
Gene ID (NCBI) 9575
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Circadian locomoter output cycles protein kaput (CLOCK), also named as BHLHE8 or KIAA0334, is a 846 amino acid protein, which contains one bHLH domain, one PAC domain, and two PAS domains. CLOCK localizes in the nucleus and cytoplasm. CLOCK) is expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. ARNTL/2-CLOCK heterodimers activate E-box element (5'-CACGTG-3') transcription of a number of proteins of the circadian clock, such as PER1 and PER2. The calculated molecular weight of CLOCK is 95kDa, we detected a 95-110 kDa protein by western blot. Our result is similar to the result of reseach paper (PMID: 30683868).