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Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using CLPP Monoclonal antibody (66271-1-Ig)

    WB analysis of human heart using 66271-1-Ig (same clone as 66271-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 66271-1-Ig (CLPP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66271-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig heart tissue using CLPP Monoclonal antibody (66271-1-Ig)

    WB analysis of pig heart using 66271-1-Ig (same clone as 66271-1-PBS)

    pig heart tissue were subjected to SDS PAGE followed by western blot with 66271-1-Ig (CLPP Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66271-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using CLPP Monoclonal antibody (66271-1-Ig)

    IHC staining of human heart using 66271-1-Ig (same clone as 66271-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66271-1-Ig (CLPP Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66271-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

CLPP Monoclonal antibody, PBS Only

CLPP Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66271-1-PBS
Clone No.2E1D9

Host / Isotype

Mouse / IgG3

Reactivity

human, pig

Applications

WB, IHC, Indirect ELISA

Synonyms

CLPP, Endopeptidase Clp

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66271-1-PBS targets CLPP in WB, IHC, Indirect ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen CLPP fusion protein Ag8420 相同性解析による交差性が予測される生物種
Full Name ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog (E. coli)
Calculated molecular weight 30 kDa
Observed molecular weight 26 kDa, 30 kDa
GenBank accession numberBC002956
Gene Symbol CLPP
Gene ID (NCBI) 8192
RRIDAB_2881656
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ16740
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CLPP is the putative ATP-dependent Clp protease proteolytic subunit and also named as endopeptidase Clp. It belongs to the peptidase S14 family. Clp cleaves peptides in various proteins in a process that requires ATP hydrolysis. It may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates. This protein has a transit peptide of 56 amino acid.