Claspin Polyclonal antibody
Claspin Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 23206-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
Product Information
23206-1-AP targets Claspin in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Claspin fusion protein Ag19637 相同性解析による交差性が予測される生物種 |
| Full Name | claspin homolog (Xenopus laevis) |
| Calculated molecular weight | 1339 aa, 151 kDa |
| Observed molecular weight | 240 kDa |
| GenBank accession number | BC137279 |
| Gene symbol | Claspin |
| Gene ID (NCBI) | 63967 |
| RRID | AB_2879232 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CLSPN, also named as Claspin, is a 1339 amino acid protein, which belongs to the claspin family. CLSPN localizes in the nucleus and is highly expressed at S/G2 phases of the cell cycle. CLSPN is required for checkpoint mediated cell cycle arrest in response to inhibition of DNA replication or to DNA damage induced by both ionizing and UV irradiation. CLSPN plays a potentially critical role in replication checkpoint control in cells and mediating phosphorylation and activation of checkpoint protein kinases. The calculated molecular weight of CLSPN is 151 kDa, but the modified protein is about 220-240 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Claspin antibody 23206-1-AP | Download protocol |
| IHC protocol for Claspin antibody 23206-1-AP | Download protocol |
| IF protocol for Claspin antibody 23206-1-AP | Download protocol |
| IP protocol for Claspin antibody 23206-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Mol Life Sci HAS3-induced extracellular vesicles from melanoma cells stimulate IHH mediated c-Myc upregulation via the hedgehog signaling pathway in target cells. | ||
Int J Oncol Deubiquitinating enzyme USP20 is a positive regulator of Claspin and suppresses the malignant characteristics of gastric cancer cells. | ||
Cell Insight ATR/Chk1 interacting lncRNA modulates DNA damage response to induce breast cancer chemoresistance |
