Validation Data Gallery
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Tested Applications
| Positive WB detected in | SH-SY5Y cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 1 publications below |
Product Information
21436-1-AP targets CLSTN2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CLSTN2 fusion protein Ag16048 相同性解析による交差性が予測される生物種 |
| Full Name | calsyntenin 2 |
| Calculated molecular weight | 955 aa, 107 kDa |
| Observed molecular weight | 100-107 kDa |
| GenBank accession number | BC104485 |
| Gene Symbol | CLSTN2 |
| Gene ID (NCBI) | 64084 |
| RRID | AB_3085656 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H4D0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CLSTN2 gene encodes the synaptic protein calsyntenin 2, one of the three calsyntenin family members found in humans. CLSTN2 is expressed exclusively in the brain, with high levels occurring in cortical gamma-aminobutyric acid (GABA) interneurons and in medial temporal lobe regions (PMID: 19058786). Western blot analysis detected CLSTN2 at an apparent molecular mass of 107 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CLSTN2 antibody 21436-1-AP | Download protocol |
| IHC protocol for CLSTN2 antibody 21436-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Clin Transl Med EHMT2-mediated R-loop formation promotes the malignant progression of prostate cancer via activating Aurora B |