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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CLSTN3 Recombinant antibody (83931-1-RR)

    WB analysis using 83931-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83931-1-RR (CLSTN3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83931-1-RR

    Affinity and Kinetic Characterization of 83931-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83931-1-RR against Human CLSTN3 were performed. The affinity constant is 1.51 nM.

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CLSTN3 Recombinant antibody

CLSTN3 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83931-1-RR
Clone No.240865C10

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Synonyms

Alc-beta, alcbeta, Alcadein-beta, Alcadein beta, Alc beta

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inrat brain tissue, rat cerebellum tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83931-1-RR targets CLSTN3 in WB, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CLSTN3 fusion protein Ag14897 相同性解析による交差性が予測される生物種
Full Name calsyntenin 3
Calculated molecular weight 968 aa, 107 kDa
Observed molecular weight130 kDa, 140 kDa
GenBank accession numberBC039075
Gene Symbol CLSTN3
Gene ID (NCBI) 9746
RRIDAB_3671512
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9BQT9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Calsyntenins, also called alcadeins, are cadherin superfamily proteins first identified as synaptic proteins (PMID: 11161476; 12498782). Calsyntenins are type I transmembrane proteins with extracellular domains containing two cadherin repeats and an LNS (laminin, neurexin, sex hormone-binding globulin) domain (PMID: 24613359). Calsyntenin-3 (CLSTN3, also known as alcadein-beta) is a synapse-organizing protein that promotes the development of synapses. It is a postsynaptic adhesion molecule that binds to presynaptic neurexins to mediate both excitatory and inhibitory synapse formation (PMID: 25352602; 24613359).

Protocols

Product Specific Protocols
WB protocol for CLSTN3 antibody 83931-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols