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Validation Data Gallery

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  • Western Blot (WB) analysis of pig brain tissue using CLTB Monoclonal antibody (66270-1-Ig)

    WB analysis of pig brain using 66270-1-Ig (same clone as 66270-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66270-1-Ig (CLTB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66270-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using CLTB Monoclonal antibody (66270-1-Ig)

    IHC staining of human liver using 66270-1-Ig (same clone as 66270-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66270-1-Ig (CLTB Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66270-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using CLTB Monoclonal antibody (66270-1-Ig)

    IHC staining of human liver using 66270-1-Ig (same clone as 66270-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66270-1-Ig (CLTB Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66270-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CLTB Monoclonal antibody (66270-1-Ig)

    IHC staining of human kidney using 66270-1-Ig (same clone as 66270-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66270-1-Ig (CLTB Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66270-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CLTB Monoclonal antibody (66270-1-Ig)

    IHC staining of human kidney using 66270-1-Ig (same clone as 66270-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66270-1-Ig (CLTB Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66270-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CLTB Monoclonal antibody (66270-1-Ig)

    IF Staining of HeLa using 66270-1-Ig (same clone as 66270-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66270-1-Ig(CLTB antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66270-1-PBS in a different storage buffer formulation.

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CLTB Monoclonal antibody, PBS Only

CLTB Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66270-1-PBS
Clone No.1F9C2

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Clathrin light chain B, clathrin, light chain (Lcb), CLTB, LCB

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66270-1-PBS targets CLTB in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CLTB fusion protein Ag24347 相同性解析による交差性が予測される生物種
Full Name clathrin, light chain (Lcb)
Calculated molecular weight 23 kDa
Observed molecular weight 32-38 kDa
GenBank accession numberBC006457
Gene Symbol CLTB
Gene ID (NCBI) 1212
RRIDAB_2881655
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP09497
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. The clathrin molecule has a triskelion shape. Each clathrin triskelion is composed of three identical heavy chains (180 kDa) and three light chains of two types, LCA (CLTA) and LCB (CLTB) (30-40 kDa). The light chain subunits are thought to regulate the formation or disassembly of clathrin coats. (PMID: 2445759; 8374173; 3563513)