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  • Featured Product
  • KD/KO Validated

CLTC Polyclonal antibody

CLTC Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 26523-1-AP

Synonyms

CLH 17, clathrin, heavy chain (Hc), Clathrin heavy chain 1, Clathrin heavy chain, CHC17

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CLTC Polyclonal antibody (26523-1-AP)

    WB analysis of HeLa using 26523-1-AP

    WB result of CLTC antibody (26523-1-AP; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CLTC transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using CLTC Polyclonal antibody (26523-1-AP)

    WB analysis using 26523-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26523-1-AP (CLTC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using CLTC Polyclonal antibody (26523-1-AP)

    WB analysis of pig brain using 26523-1-AP

    pig brain tissue were subjected to SDS PAGE followed by western blot with 26523-1-AP (CLTC Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using CLTC Polyclonal antibody (26523-1-AP)

    WB analysis of Raji using 26523-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 26523-1-AP (CLTC Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using CLTC Polyclonal antibody (26523-1-AP)

    WB analysis of HL-60 using 26523-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 26523-1-AP (CLTC Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using CLTC Polyclonal antibody (26523-1-AP)

    IP experiment of HeLa using 26523-1-AP

    IP result of anti-CLTC (IP:26523-1-AP, 4ug; Detection:26523-1-AP 1:500) with HeLa cells lysate 2500ug.

  • Immunohistochemistry (IHC) staining of human colon tissue using CLTC Polyclonal antibody (26523-1-AP)

    IHC staining of human colon using 26523-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 26523-1-AP (CLTC antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CLTC Polyclonal antibody (26523-1-AP)

    IHC staining of human breast cancer using 26523-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26523-1-AP (CLTC Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CLTC Polyclonal antibody (26523-1-AP)

    IHC staining of human breast cancer using 26523-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26523-1-AP (CLTC Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CLTC Polyclonal antibody (26523-1-AP)

    IHC staining of human kidney using 26523-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26523-1-AP (CLTC Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CLTC Polyclonal antibody (26523-1-AP)

    IHC staining of human kidney using 26523-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26523-1-AP (CLTC Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CLTC Polyclonal antibody (26523-1-AP)

    IF Staining of HepG2 using 26523-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CLTC antibody (26523-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CLTC Polyclonal antibody (26523-1-AP)

    IF Staining of HeLa using 26523-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CLTC antibody (26523-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using CLTC Polyclonal antibody (26523-1-AP)

    FC experiment of HeLa using 26523-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human CLTC (26523-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inHeLa cells, HL-60 cells, pig brain tissue, Raji cells, mouse brain tissue, rat brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon tissue, human breast cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26523-1-AP targets CLTC in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CLTC fusion protein Ag25041 相同性解析による交差性が予測される生物種
Full Name clathrin, heavy chain (Hc)
Calculated molecular weight 192 kDa
Observed molecular weight 180 kDa
GenBank accession numberBC054489
Gene symbol CLTC
Gene ID (NCBI) 1213
RRIDAB_2880542
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. The clathrin molecule has a triskelion shape. Each clathrin triskelion is composed of three identical heavy chains (CLTC) (160-190 kDa) and three light chains of two types, LCA (CLTA) and LCB (CLTB) (30-40 kDa). The heavy chain provides the structural backbone of the clathrin lattice. (PMID: 9133677)

Protocols

Product Specific Protocols
WB protocol for CLTC antibody 26523-1-APDownload protocol
IHC protocol for CLTC antibody 26523-1-APDownload protocol
IF protocol for CLTC antibody 26523-1-APDownload protocol
IP protocol for CLTC antibody 26523-1-APDownload protocol
FC protocol for CLTC antibody 26523-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Emerg Microbes Infect

CD1d facilitates African swine fever virus entry into the host cells via clathrin-mediated endocytosis

Authors - Xin Chen
View Article
humanWB

Autophagy

AP2M1 mediates autophagy-induced CLDN2 (claudin 2) degradation through endocytosis and interaction with LC3 and reduces intestinal epithelial tight junction permeability.

Authors - Ashwinkumar Subramenium Ganapathy
View Article
humanIF

Br J Pharmacol

A glycine-serine-alanine-rich peptide ameliorates Alzheimer-type neurodegeneration

Authors - Si-Yuan Yao
View Article
mouseIF

Aging (Albany NY)

The interaction protein of SORBS2 in myocardial tissue to find out the pathogenic mechanism of LVNC disease.

Authors - Chunyan Li
View Article
bovineWB,IF

Vet Microbiol

Invasion of Mycoplasma bovis into bovine synovial cells utilizing the clathrin-dependent endocytosis pathway.

Authors - Koji Nishi
  • KD Validated
View Article
humanIF

FASEB J

A genome-wide transposon mutagenesis screening identifies LppB as a key factor associated with Mycoplasma bovis colonization and invasion into host cells

Authors - Shimei Lan
View Article