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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CLTC Monoclonal antibody (66487-1-Ig)

    WB analysis using 66487-1-Ig (same clone as 66487-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66487-1-Ig (CLTC antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using CLTC Monoclonal antibody (66487-1-Ig)

    WB analysis of HeLa using 66487-1-Ig (same clone as 66487-1-PBS)

    WB result of CLTC antibody (66487-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CLTC transfected HeLa cells. This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CLTC Monoclonal antibody (66487-1-Ig)

    WB analysis using 66487-1-Ig (same clone as 66487-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66487-1-Ig (CLTC antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using CLTC Monoclonal antibody (66487-1-Ig)

    WB analysis of HeLa using 66487-1-Ig (same clone as 66487-1-PBS)

    HeLa, Jurkat, ROS1728 cells, and pig brain tissue were subjected to SDS PAGE followed by western blot with 66487-1-Ig (CLTC antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using CLTC Monoclonal antibody (66487-1-Ig)

    WB analysis of Raji using 66487-1-Ig (same clone as 66487-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 66487-1-Ig (CLTC antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using CLTC Monoclonal antibody (66487-1-Ig)

    IHC staining of mouse kidney using 66487-1-Ig (same clone as 66487-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66487-1-Ig (CLTC antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using CLTC Monoclonal antibody (66487-1-Ig)

    IHC staining of mouse kidney using 66487-1-Ig (same clone as 66487-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66487-1-Ig (CLTC antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using CLTC Monoclonal antibody (66487-1-Ig)

    IHC staining of mouse kidney using 66487-1-Ig (same clone as 66487-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66487-1-Ig (CLTC antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using CLTC Monoclonal antibody (66487-1-Ig)

    IHC staining of mouse kidney using 66487-1-Ig (same clone as 66487-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66487-1-Ig (CLTC antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CLTC Monoclonal antibody (66487-1-Ig)

    IHC staining of human breast cancer using 66487-1-Ig (same clone as 66487-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66487-1-Ig (CLTC antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CLTC Monoclonal antibody (66487-1-Ig)

    IHC staining of human breast cancer using 66487-1-Ig (same clone as 66487-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66487-1-Ig (CLTC antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using CLTC Monoclonal antibody (66487-1-Ig)

    IHC staining of mouse kidney using 66487-1-Ig (same clone as 66487-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66487-1-Ig (CLTC antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using CLTC Monoclonal antibody (66487-1-Ig)

    IHC staining of mouse kidney using 66487-1-Ig (same clone as 66487-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66487-1-Ig (CLTC antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CLTC Monoclonal antibody (66487-1-Ig)

    IF Staining of HepG2 using 66487-1-Ig (same clone as 66487-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CLTC antibody (66487-1-Ig, Clone: 1B3D7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CLTC Monoclonal antibody (66487-1-Ig)

    IF Staining of HeLa using 66487-1-Ig (same clone as 66487-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66487-1-Ig (CLTC antibody), at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

CLTC Monoclonal antibody, PBS Only

CLTC Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66487-1-PBS
Clone No.1B3D7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

clathrin, heavy chain (Hc), Clathrin heavy chain 1, Clathrin heavy chain, CHC17, CHC

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66487-1-PBS targets CLTC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CLTC fusion protein Ag25040 相同性解析による交差性が予測される生物種
Full Name clathrin, heavy chain (Hc)
Calculated molecular weight 192 kDa
Observed molecular weight 170 kDa
GenBank accession numberBC054489
Gene Symbol CLTC
Gene ID (NCBI) 1213
RRIDAB_2881852
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ00610
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. The clathrin molecule has a triskelion shape. Each clathrin triskelion is composed of three identical heavy chains (CLTC) (160-190 kDa) and three light chains of two types, LCA (CLTA) and LCB (CLTB) (30-40 kDa). The heavy chain provides the structural backbone of the clathrin lattice. (PMID: 9133677)