Validation Data Gallery
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Tested Applications
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human testis tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IF | See 2 publications below |
| IP | See 1 publications below |
Product Information
22283-1-AP targets CLTCL1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | clathrin, heavy chain-like 1 |
| Calculated molecular weight | 187 kDa |
| Observed molecular weight | 180-210 kDa |
| GenBank accession number | NM_007098 |
| Gene Symbol | CLTCL1 |
| Gene ID (NCBI) | 8218 |
| RRID | AB_11183764 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P53675 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CLTCL1, also called CLH22, CLTCL, and CLTD, belongs to the clathrin heavy chain family. Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. The antibody is specific to CLTCL1. It has no cross-reaction to CLTC.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CLTCL1 antibody 22283-1-AP | Download protocol |
| IF protocol for CLTCL1 antibody 22283-1-AP | Download protocol |
| IP protocol for CLTCL1 antibody 22283-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EMBO J CHC22 clathrin recruitment to the early secretory pathway requires two-site interaction with SNX5 and p115 | ||
J Cell Biol CHC22 clathrin mediates traffic from early secretory compartments for human GLUT4 pathway biogenesis.
| ||
Proteome Sci LC-MS-based quantitation of proteomic changes induced by Norcantharidin in MTB-Treated macrophages |