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CLUAP1 Polyclonal antibody
CLUAP1 Polyclonal Antibody for WB, IP, IF, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, Canine
Applications
WB, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 17470-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse testis tissue, COLO 320 cells, HepG2 cells, Y79 cells |
Positive IP detected in | HepG2 cells |
Positive IF/ICC detected in | MDCK cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 2 publications below |
IF | See 1 publications below |
Product Information
17470-1-AP targets CLUAP1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, Canine samples.
Tested Reactivity | human, mouse, rat, Canine |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CLUAP1 fusion protein Ag11419 相同性解析による交差性が予測される生物種 |
Full Name | clusterin associated protein 1 |
Calculated molecular weight | 413 aa, 48 kDa |
Observed molecular weight | 53 kDa |
GenBank accession number | BC017070 |
Gene symbol | CLUAP1 |
Gene ID (NCBI) | 23059 |
RRID | AB_2083477 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CLUAP1 (Clusterin-associated protein 1) was originally identified as a protein that interacts with CLU/clusterin, and whose gene is frequently upregulated in colon cancer (PMID: 15480429). Overexpression of CLUAP1 was also observed in osteosarcoma (PMID: 17203229). CLUAP1 is most abundantly expressed in testis among normal tissues (PMID: 15480429; 17203229). Studies reveal that CLUAP1 has a role in ciliogenenesis and cilia-dependent development (PMID: 23351563; 23742838; 24970261).
Protocols
Product Specific Protocols | |
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WB protocol for CLUAP1 antibody 17470-1-AP | Download protocol |
IF protocol for CLUAP1 antibody 17470-1-AP | Download protocol |
IP protocol for CLUAP1 antibody 17470-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Dev Cell The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base. | ||
Mol Cell Proteomics CRISPR/Cas9-mediated genomic editing of Cluap1/IFT38 reveals a new role in actin arrangement.
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