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  • Featured Product
  • KD/KO Validated

CLUAP1 Polyclonal antibody

CLUAP1 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, Canine

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 17470-1-AP

Synonyms

CLUAP1, clusterin associated protein 1, KIAA0643, Qilin

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using CLUAP1 Polyclonal antibody (17470-1-AP)

    WB analysis of mouse testis using 17470-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 17470-1-AP (CLUAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using CLUAP1 Polyclonal antibody (17470-1-AP)

    WB analysis of COLO 320 using 17470-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 17470-1-AP (CLUAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using CLUAP1 Polyclonal antibody (17470-1-AP)

    WB analysis of HepG2 using 17470-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 17470-1-AP (CLUAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using CLUAP1 Polyclonal antibody (17470-1-AP)

    WB analysis of Y79 using 17470-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 17470-1-AP (CLUAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using CLUAP1 Polyclonal antibody (17470-1-AP)

    WB analysis of HepG2 using 17470-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 17470-1-AP (CLUAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using CLUAP1 Polyclonal antibody (17470-1-AP)

    IP experiment of HepG2 using 17470-1-AP

    IP result of anti-CLUAP1 (IP:17470-1-AP, 4ug; Detection:17470-1-AP 1:600) with HepG2 cells lysate 2400ug.

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using CLUAP1 Polyclonal antibody (17470-1-AP)

    IF Staining of MDCK using 17470-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CLUAP1 antibody (17470-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594 ARL13B antibody (CL594-17711, red).

Tested Applications

Positive WB detected inmouse testis tissue, COLO 320 cells, HepG2 cells, Y79 cells
Positive IP detected inHepG2 cells
Positive IF/ICC detected inMDCK cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17470-1-AP targets CLUAP1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, Canine samples.

Tested Reactivity human, mouse, rat, Canine
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CLUAP1 fusion protein Ag11419 相同性解析による交差性が予測される生物種
Full Name clusterin associated protein 1
Calculated molecular weight 413 aa, 48 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC017070
Gene symbol CLUAP1
Gene ID (NCBI) 23059
RRIDAB_2083477
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CLUAP1 (Clusterin-associated protein 1) was originally identified as a protein that interacts with CLU/clusterin, and whose gene is frequently upregulated in colon cancer (PMID: 15480429). Overexpression of CLUAP1 was also observed in osteosarcoma (PMID: 17203229). CLUAP1 is most abundantly expressed in testis among normal tissues (PMID: 15480429; 17203229). Studies reveal that CLUAP1 has a role in ciliogenenesis and cilia-dependent development (PMID: 23351563; 23742838; 24970261).

Protocols

Product Specific Protocols
WB protocol for CLUAP1 antibody 17470-1-APDownload protocol
IF protocol for CLUAP1 antibody 17470-1-APDownload protocol
IP protocol for CLUAP1 antibody 17470-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Dev Cell

The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base.

Authors - Tomoharu Kanie
View Article
humanWB,IF

Mol Cell Proteomics

CRISPR/Cas9-mediated genomic editing of Cluap1/IFT38 reveals a new role in actin arrangement.

Authors - Tina Beyer
  • KO Validated
View Article