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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CLUH Polyclonal antibody (30986-1-AP)

    WB analysis using 30986-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30986-1-AP (CLUH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of COS-7 cells using CLUH Polyclonal antibody (30986-1-AP)

    IF Staining of COS-7 using 30986-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed COS-7 cells using CLUH antibody (30986-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

CLUH Polyclonal antibody

CLUH Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30986-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, monkey

Applications

WB, IF/ICC, ELISA

Synonyms

KIAA0664, Protein KIAA0664, FLJ23672, CLU1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, mouse heart tissue, mouse kidney tissue
Positive IF/ICC detected inCOS-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30986-1-AP targets CLUH in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, monkey samples.

Tested Reactivity human, mouse, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CLUH fusion protein Ag34275 相同性解析による交差性が予測される生物種
Full Name KIAA0664
Observed molecular weight147-150 kDa
GenBank accession numberBC156940
Gene Symbol CLUH
Gene ID (NCBI) 23277
RRIDAB_3669806
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDO75153
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Clustered mitochondria homolog (CLUH) is a conserved RNA-binding protein, that binds transcripts encoding proteins involved in the respiratory chain, the tricarboxylic acid (TCA) cycle, and other mitochondrial metabolic pathways. Upon loss of CLUH, target mRNAs are subjected to faster decay, and their respective proteins are decreased in abundance. This leads to alterations in mitochondrial distribution (mitochondrial clustering as the gene name alludes to) mitochondrial cristae integrity, respiratory defects, loss of mtDNA, and decreased activity of TCA cycle enzymes. (PMID: 35559794)

Protocols

Product Specific Protocols
WB protocol for CLUH antibody 30986-1-APDownload protocol
IF protocol for CLUH antibody 30986-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols