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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CMAS Polyclonal antibody

CMAS Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 13006-1-AP

Synonyms

CMAS, CMP Neu5Ac SYNTHETASE, CMP NeuNAc synthase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CMAS Polyclonal antibody (13006-1-AP)

    WB analysis using 13006-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13006-1-AP (CMAS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CMAS Polyclonal antibody (13006-1-AP)

    WB analysis using 13006-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13006-1-AP (CMAS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inrat pancreas tissue, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13006-1-AP targets CMAS in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CMAS fusion protein Ag3686 相同性解析による交差性が予測される生物種
Full Name cytidine monophosphate N-acetylneuraminic acid synthetase
Calculated molecular weight 48 kDa
Observed molecular weight48 kDa
GenBank accession numberBC016609
Gene symbol CMAS
Gene ID (NCBI) 55907
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cytidine monophosphate N-acetylneuraminic acid synthetase (CMAS), a key enzyme in the incorporation pathway, catalyzes the synthesis of CMP-sialic acid by utilizing CTP and sialic acid. Neu5Ac is activated in the nucleus by CMAS to form CMP-Neu5Ac, which is transported to the Golgi apparatus by SLC35A1 for sialylation of glycoproteins and gangliosides catalyzed by specific sialy ltransferases (PMID: 30568043). Low CMAS gene expression correlated with reduced recurrence-free survival in a human colorectal cancer cohort (PMID: 30578646). Western blots indicated a range of bands at ~ 48 kDa.

Protocols

Product Specific Protocols
WB protocol for CMAS antibody 13006-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols