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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CMAS Polyclonal antibody (13006-1-AP)

    WB analysis using 13006-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13006-1-AP (CMAS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CMAS Polyclonal antibody (13006-1-AP)

    WB analysis using 13006-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13006-1-AP (CMAS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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CMAS Polyclonal antibody

CMAS Polyclonal Antibody for WB, ELISA
Cat No. 13006-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

CMAS, CMP Neu5Ac SYNTHETASE, CMP NeuNAc synthase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inrat pancreas tissue, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13006-1-AP targets CMAS in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CMAS fusion protein Ag3686 相同性解析による交差性が予測される生物種
Full Name cytidine monophosphate N-acetylneuraminic acid synthetase
Calculated molecular weight 48 kDa
Observed molecular weight48 kDa
GenBank accession numberBC016609
Gene Symbol CMAS
Gene ID (NCBI) 55907
RRIDAB_3085404
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NFW8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cytidine monophosphate N-acetylneuraminic acid synthetase (CMAS), a key enzyme in the incorporation pathway, catalyzes the synthesis of CMP-sialic acid by utilizing CTP and sialic acid. Neu5Ac is activated in the nucleus by CMAS to form CMP-Neu5Ac, which is transported to the Golgi apparatus by SLC35A1 for sialylation of glycoproteins and gangliosides catalyzed by specific sialy ltransferases (PMID: 30568043). Low CMAS gene expression correlated with reduced recurrence-free survival in a human colorectal cancer cohort (PMID: 30578646). Western blots indicated a range of bands at ~ 48 kDa.

Protocols

Product Specific Protocols
WB protocol for CMAS antibody 13006-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols