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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CMC1 Polyclonal antibody

CMC1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24030-1-AP

Synonyms

C3orf68, CMC1, Cmc1p

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CMC1 Polyclonal antibody (24030-1-AP)

    WB analysis using 24030-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24030-1-AP (CMC1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using CMC1 Polyclonal antibody (24030-1-AP)

    IP experiment of K-562 using 24030-1-AP

    IP result of anti-CMC1 (IP:24030-1-AP, 4ug; Detection:24030-1-AP 1:300) with K-562 cells lysate 4000 ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CMC1 Polyclonal antibody (24030-1-AP)

    IHC staining of human liver cancer using 24030-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24030-1-AP (CMC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CMC1 Polyclonal antibody (24030-1-AP)

    IHC staining of human liver cancer using 24030-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24030-1-AP (CMC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inK-562 cells, mouse liver tissue
Positive IP detected inK-562 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 24030-1-AP is CMC1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CMC1 fusion protein Ag21224 相同性解析による交差性が予測される生物種
Full Name COX assembly mitochondrial protein homolog (S. cerevisiae)
Calculated molecular weight 106 aa, 12 kDa
Observed molecular weight 12-15 kDa
GenBank accession numberBC052644
Gene symbol CMC1
Gene ID (NCBI) 152100
RRIDAB_2879409
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CMC1 antibody 24030-1-APDownload protocol
IHC protocol for CMC1 antibody 24030-1-APDownload protocol
IP protocol for CMC1 antibody 24030-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Oncol

A novel defined cuproptosis-related gene signature for predicting the prognosis of colon adenocarcinoma

Authors - Bixian Luo
View Article