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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CMIP Polyclonal antibody

CMIP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 31407-1-AP

Synonyms

TCMIP, Tc Mip, c-Mip, C-Maf-inducing protein, c Mip

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CMIP Polyclonal antibody (31407-1-AP)

    WB analysis using 31407-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31407-1-AP (CMIP antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using CMIP Polyclonal antibody (31407-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 31407-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 31407-1-AP (CMIP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using CMIP Polyclonal antibody (31407-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 31407-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 31407-1-AP (CMIP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inRaji cells, MCF-7 cells, K-562 cells, A549 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31407-1-AP targets CMIP in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CMIP fusion protein Ag35679 相同性解析による交差性が予測される生物種
Full Name c-Maf-inducing protein
Calculated molecular weight 86 kDa
Observed molecular weight86 kDa
GenBank accession numberBC038113
Gene symbol CMIP
Gene ID (NCBI) 80790
RRIDAB_3669968
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cmaf‐inducing protein (CMIP) is a newly identified gene that encodes an 86 kDa protein, containing an N‐terminal pleckstrin homology domain (PH), a nuclear localization signal near the PH domain, a middle region characterized by the presence of several interacting docking sites, including a 14‐3‐3 module, a PKC domain, an Erk domain, an SH3 domain similar to the p85 regulatory subunit of phosphatidylinositol 3‐kinase (PI3K), and a C‐terminal leucine‐rich repeat (LRR) domain.

Protocols

Product Specific Protocols
WB protocol for CMIP antibody 31407-1-APDownload protocol
IHC protocol for CMIP antibody 31407-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols