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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CMPK1 Monoclonal antibody, PBS Only

CMPK1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2D11G7

Cat no : 67519-1-PBS

Synonyms

CMK, CMPK, CMPK1, Cytidine monophosphate kinase, Cytidylate kinase, Deoxycytidylate kinase, UCK, UMK, UMP CMP kinase, UMP CMPK, UMP/CMP kinase, UMP/CMPK, UMPK, Uridine monophosphate kinase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CMPK1 Monoclonal antibody (67519-1-Ig)

    WB analysis using 67519-1-Ig (same clone as 67519-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67519-1-Ig (CMPK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67519-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CMPK1 Monoclonal antibody (67519-1-Ig)

    IF Staining of HeLa using 67519-1-Ig (same clone as 67519-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CMPK1 antibody (67519-1-Ig, Clone: 2D11G7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67519-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67519-1-PBS targets CMPK1 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CMPK1 fusion protein Ag30084 相同性解析による交差性が予測される生物種
Full Name cytidine monophosphate (UMP-CMP) kinase 1, cytosolic
Calculated molecular weight 228 aa, 26 kDa
Observed molecular weight 22-26 kDa
GenBank accession numberBC014961
Gene symbol CMPK1
Gene ID (NCBI) 51727
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

Pyrimidine nucleoside monophosphate kinase [UMP/CMP kinase (UMP/CMPK)] (CMPK1) is also named as CMK, CMPK, UCK, UMK, UMPK and belongs to the adenylate kinase family. It plays a crucial role in the formation of UDP, CDP, and dCDP, which are required for cellular nucleic acid synthesis(PMID:11912132). CMPK1 can use a broad spectrum of phosphate donors but the best phosphate donors are ATP and dATP. CMPK1 has been reported as a prognostic marker for non-small cell lung cancer, pancreatic cancer and breast cancer (PMID: 21642870, 22838950, 27558661). The longest deduced protein contains 228 amino acids and has a calculated molecular mass of 26 kD. However, translation likely begins at the second initiation methionine, resulting in a protein of 196 amino acids(PMID:10462544). The shorter form is the endogenously translated protein(PMID:11912132). CMPK1 has 3 isoforms with the molecular mass of 22, 16 and 26 kDa.