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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CNBP Polyclonal antibody

CNBP Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12103-1-AP

Synonyms

RNF163, PROMM, DM2, CNBP1, Cellular nucleic acid-binding protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CNBP Polyclonal antibody (12103-1-AP)

    WB analysis using 12103-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12103-1-AP (CNBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CNBP Polyclonal antibody (12103-1-AP)

    IF Staining of HeLa using 12103-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CNBP antibody (12103-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inHeLa cells, NIH/3T3 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12103-1-AP targets CNBP in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CNBP fusion protein Ag2747 相同性解析による交差性が予測される生物種
Full Name CCHC-type zinc finger, nucleic acid binding protein
Calculated molecular weight 170 aa, 19 kDa
Observed molecular weight21 kDa
GenBank accession numberBC014911
Gene symbol CNBP
Gene ID (NCBI) 7555
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cellular nucleic acid binding protein (CNBP) has been implicated in vertebrate craniofacial development and in myotonic dystrophy type 2 (DM2) and sporadic inclusion body myositis (sIBM) human diseases [PMID:21277287]. CNBP is a small and strikingly conserved single-stranded nucleic acid binding protein that is able to bind DNA as well as RNA. This ssDNA- and RNA-binding protein with broad sequence specificity is participated in diverse cellular functions, including transcription, translation and through them cell proliferation and survival [PMID:20960530].

Protocols

Product Specific Protocols
WB protocol for CNBP antibody 12103-1-APDownload protocol
IF protocol for CNBP antibody 12103-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols