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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

CNBP Polyclonal antibody

CNBP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA, IF

Conjugate

Unconjugated

Cat no : 14717-1-AP

Synonyms

RNF163, PROMM, DM2, CNBP1, Cellular nucleic acid-binding protein

Validation Data Gallery

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  • Western Blot (WB) analysis of Caco-2 cells using CNBP Polyclonal antibody (14717-1-AP)

    WB analysis of Caco-2 using 14717-1-AP

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 14717-1-AP (CNBP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CNBP Polyclonal antibody (14717-1-AP)

    WB analysis using 14717-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14717-1-AP (CNBP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using CNBP Polyclonal antibody (14717-1-AP)

    WB analysis of mouse heart using 14717-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 14717-1-AP (CNBP Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of HeLa cells using CNBP Polyclonal antibody (14717-1-AP)

    WB analysis of HeLa using 14717-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14717-1-AP (CNBP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using CNBP Polyclonal antibody (14717-1-AP)

    WB analysis of mouse testis using 14717-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 14717-1-AP (CNBP Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tissue using CNBP Polyclonal antibody (14717-1-AP)

    IHC staining of human ovary using 14717-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using CNBP Polyclonal antibody (14717-1-AP)

    IHC staining of human ovary using 14717-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using CNBP Polyclonal antibody (14717-1-AP)

    IHC staining of human lung using 14717-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using CNBP Polyclonal antibody (14717-1-AP)

    IHC staining of human lung using 14717-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using CNBP Polyclonal antibody (14717-1-AP)

    IHC staining of human heart using 14717-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using CNBP Polyclonal antibody (14717-1-AP)

    IHC staining of human heart using 14717-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CNBP Polyclonal antibody (14717-1-AP)

    IHC staining of human kidney using 14717-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CNBP Polyclonal antibody (14717-1-AP)

    IHC staining of human kidney using 14717-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using CNBP Polyclonal antibody (14717-1-AP)

    IHC staining of human testis using 14717-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using CNBP Polyclonal antibody (14717-1-AP)

    IHC staining of human testis using 14717-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using CNBP Polyclonal antibody (14717-1-AP)

    IHC staining of human skin using 14717-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using CNBP Polyclonal antibody (14717-1-AP)

    IHC staining of human skin using 14717-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using CNBP Polyclonal antibody (14717-1-AP)

    IHC staining of human brain using 14717-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using CNBP Polyclonal antibody (14717-1-AP)

    IHC staining of human brain using 14717-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using CNBP Polyclonal antibody (14717-1-AP)

    IHC staining of human spleen using 14717-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using CNBP Polyclonal antibody (14717-1-AP)

    IHC staining of human spleen using 14717-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 14717-1-AP (CNBP antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inCaco-2 cells, HeLa cells, mouse heart tissue, mouse testis tissue, U-251 cells
Positive IHC detected inhuman ovary tissue, human brain tissue, human heart tissue, human kidney tissue, human lung tissue, human skin tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14717-1-AP targets CNBP in WB, IHC, ELISA, IF applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CNBP fusion protein Ag6441 相同性解析による交差性が予測される生物種
Full Name CCHC-type zinc finger, nucleic acid binding protein
Calculated molecular weight 19 kDa
Observed molecular weight 20-25 kDa
GenBank accession numberBC000288
Gene symbol CNBP
Gene ID (NCBI) 7555
RRIDAB_2081548
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Single stranded DNA-binding protein, with specificity to the sterol regulatory element (SRE). Involved in sterol-mediated repression.

Protocols

Product Specific Protocols
WB protocol for CNBP antibody 14717-1-APDownload protocol
IHC protocol for CNBP antibody 14717-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS Biol

Phosphoglucomutase 1 inhibits hepatocellular carcinoma progression by regulating glucose trafficking.

Authors - Guang-Zhi Jin
  • KD Validated
View Article
mouseWB

Mol Cell Proteomics

Quantitative proteomics reveals the essential roles of STIM1 in the testicular cord formation in mouse testis.

Authors - Bo Zheng
View Article
mouseWB,IF

iScience

Proteostasis regulated by testis-specific ribosomal protein RPL39L maintains mouse spermatogenesis.

Authors - Qianxing Zou
View Article
mouseWB,IF

Reproduction

Cellular nucleic acid-binding protein is vital to testis development and spermatogenesis in mice.

Authors - Bo Zheng
View Article