Validation Data Gallery
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Tested Applications
| Positive WB detected in | HT-29 cells, human brain tissue |
| Positive IP detected in | HT-29 cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IHC | See 3 publications below |
Product Information
10885-1-AP targets CNKSR1 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CNKSR1 fusion protein Ag1333 相同性解析による交差性が予測される生物種 |
| Full Name | connector enhancer of kinase suppressor of Ras 1 |
| Calculated molecular weight | 80 kDa |
| Observed molecular weight | 90-100 kDa |
| GenBank accession number | BC011604 |
| Gene Symbol | CNKSR1 |
| Gene ID (NCBI) | 10256 |
| RRID | AB_2276387 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q969H4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CNKSR1 is a protein containing several motifs involved in protein-protein interaction, including PDZ, PH (Pleckstrin homology), and SAM (sterile alpha motif) domains. The encoded protein acts as a scaffold component for receptor tyrosine kinase signaling and may mediate crosstalk between different signaling pathways. CNKSR1 is required in multiple receptor tyrosine kinase pathways where it appears to be a tyrosine phosphorylation targe.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CNKSR1 antibody 10885-1-AP | Download protocol |
| IHC protocol for CNKSR1 antibody 10885-1-AP | Download protocol |
| IP protocol for CNKSR1 antibody 10885-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Life Sci Alliance CNKSR1 serves as a scaffold to activate an EGFR phosphatase via exclusive interaction with RhoB-GTP.
| ||
Cancer Rep (Hoboken) Identification of hub genes in bladder cancer based on weighted gene co-expression network analysis from TCGA database. | ||
Heliyon Identification of CNKSR1 as a biomarker for "cold" tumor microenvironment in lung adenocarcinoma: An integrative analysis based on a novel workflow |