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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using CNKSR1 Monoclonal antibody (66796-1-Ig)

    WB analysis of MCF-7 using 66796-1-Ig (same clone as 66796-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66796-1-Ig (CNKSR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66796-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using CNKSR1 Monoclonal antibody (66796-1-Ig)

    WB analysis of HEK-293 using 66796-1-Ig (same clone as 66796-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66796-1-Ig (CNKSR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66796-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HT-29 cells using CNKSR1 Monoclonal antibody (66796-1-Ig)

    WB analysis of HT-29 using 66796-1-Ig (same clone as 66796-1-PBS)

    HT-29 cells were subjected to SDS PAGE followed by western blot with 66796-1-Ig (CNKSR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66796-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Caco-2 cells using CNKSR1 Monoclonal antibody (66796-1-Ig)

    WB analysis of Caco-2 using 66796-1-Ig (same clone as 66796-1-PBS)

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 66796-1-Ig (CNKSR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66796-1-PBS in a different storage buffer formulation.

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CNKSR1 Monoclonal antibody, PBS Only

CNKSR1 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 66796-1-PBS
Clone No.1E11H8

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, Indirect ELISA

Synonyms

CNK, CNK homolog protein 1, CNK1, CNKSR1, Connector enhancer of KSR 1, Connector enhancer of KSR like, hCNK1, KSR

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66796-1-PBS targets CNKSR1 in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CNKSR1 fusion protein Ag27855 相同性解析による交差性が予測される生物種
Full Name connector enhancer of kinase suppressor of Ras 1
Calculated molecular weight 80 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC011604
Gene Symbol CNKSR1
Gene ID (NCBI) 10256
RRIDAB_2882140
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ969H4
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CNKSR1 is a protein containing several motifs involved in protein-protein interaction, including PDZ, PH (Pleckstrin homology), and SAM (sterile alpha motif) domains. The encoded protein acts as a scaffold component for receptor tyrosine kinase signaling and may mediate crosstalk between different signaling pathways. CNKSR1 is required in multiple receptor tyrosine kinase pathways where it appears to be a tyrosine phosphorylation targe.