Featured Product
KD/KO Validated

CNN3 Polyclonal antibody

Name: CNN3 Polyclonal antibody
Brand: Proteintech
SKU: 11509-1-AP

CNN3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11509-1-AP

Synonyms

CNN 3, Calponin-3, Calponin3, Calponin 3

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of HEK-293 using 11509-1-AP
WB result of CNN3 antibody (11509-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNN3 transfected HEK-293 cells.
WB analysis of HAP1 cells using 11509-1-AP
HAP1 (WT and CNN3 KO) lysates prepared with RIPA buffer, 20 μg of protein loaded. 11509-1-AP incubated at 1:100 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown for each blot. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.
×
HAP1 (WT and CNN3 KO) lysates prepared with RIPA buffer, 20 μg of protein loaded. 11509-1-AP incubated at 1:100 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown for each blot. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.
WB analysis of human kidney using 11509-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 11509-1-AP (CNN3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 11509-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11509-1-AP (CNN3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of human skeletal muscle using 11509-1-AP
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11509-1-AP (CNN3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
$U-87MG lysates prepared and IP of APP performed using 2.0 μg of 11509-1-AP coupled to protein A- Dynabeads beads. The Ponceau stained transfers of each blot are shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitated; HC = antibody heavy chain. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.$
IP experiment of U-87MG cells using 11509-1-AP
U-87MG lysates prepared and IP of APP performed using 2.0 μg of 11509-1-AP coupled to protein A- Dynabeads beads. The Ponceau stained transfers of each blot are shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitated; HC = antibody heavy chain. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.
×
U-87MG lysates prepared and IP of APP performed using 2.0 μg of 11509-1-AP coupled to protein A- Dynabeads beads. The Ponceau stained transfers of each blot are shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitated; HC = antibody heavy chain. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.
IP experiment of HEK-293 using 11509-1-AP
IP result of anti-CNN3 (IP:11509-1-AP, 4ug; Detection:11509-1-AP 1:15000) with HEK-293 cells lysate 960 ug.
IHC staining of human prostate cancer using 11509-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11509-1-AP (CNN3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 11509-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11509-1-AP (CNN3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 11509-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11509-1-AP (CNN3 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin using 11509-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 11509-1-AP (CNN3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skin using 11509-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 11509-1-AP (CNN3 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human hysteromyoma using 11509-1-AP
Immunohistochemical analysis of paraffin-embedded human hysteromyoma using 11509-1-AP (CNN3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human hysteromyoma using 11509-1-AP
Immunohistochemical analysis of paraffin-embedded human hysteromyoma using 11509-1-AP (CNN3 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 11509-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CNN3 antibody (11509-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

-/ -

Cat No. 11509-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HCT 116 cells, HAP1 cells, human kidney tissue, human skeletal muscle tissue, HEK-293 cells, HepG2 cells, NIH/3T3 cells
Positive IP detected in	HEK-293 cells, U-87MG cells
Positive IHC detected in	human prostate cancer tissue, human hysteromyoma tissue, human skin tissue, human stomach cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 2 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

11509-1-AP targets CNN3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CNN3 fusion protein Ag2068 相同性解析による交差性が予測される生物種
Full Name	calponin 3, acidic
Calculated molecular weight	329 aa, 36 kDa
Observed molecular weight	36 kDa
GenBank accession number	BC025372
Gene symbol	CNN3
Gene ID (NCBI)	1266
RRID	AB_2082140
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for CNN3 antibody 11509-1-AP	Download protocol
IHC protocol for CNN3 antibody 11509-1-AP	Download protocol
IF protocol for CNN3 antibody 11509-1-AP	Download protocol
IP protocol for CNN3 antibody 11509-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Cell Rep Spatiotemporal dynamics of inner ear sensory and non-sensory cells revealed by single-cell transcriptomics. Authors - Taha A Jan View Article
human	WB	J Proteome Res Comparative Proteomics Reveals Prolonged Corneal Preservation Impaired Ocular Surface Immunity Accompanied by Fibrosis in Human Stroma Authors - Pei-Shan Wu View Article
mouse	IHC	Mol Cell Biochem STAT3 mediates ECM stiffness-dependent progression in ovarian cancer Authors - Sun Chenchen View Article
human	WB	Gastroenterol Res Pract PMID: 30911294 Authors - Keun-Seok Hong KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CNN3 Polyclonal antibody

CNN3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of HEK-293 using 11509-1-AP

WB analysis of HAP1 cells using 11509-1-AP

WB analysis of human kidney using 11509-1-AP

WB analysis using 11509-1-AP

WB analysis of human skeletal muscle using 11509-1-AP

IP experiment of U-87MG cells using 11509-1-AP

IP experiment of HEK-293 using 11509-1-AP

IHC staining of human prostate cancer using 11509-1-AP

IHC staining of human prostate cancer using 11509-1-AP

IHC staining of human stomach cancer using 11509-1-AP

IHC staining of human skin using 11509-1-AP

IHC staining of human skin using 11509-1-AP

IHC staining of human hysteromyoma using 11509-1-AP

IHC staining of human hysteromyoma using 11509-1-AP

IF Staining of HeLa using 11509-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Spatiotemporal dynamics of inner ear sensory and non-sensory cells revealed by single-cell transcriptomics.

Comparative Proteomics Reveals Prolonged Corneal Preservation Impaired Ocular Surface Immunity Accompanied by Fibrosis in Human Stroma

STAT3 mediates ECM stiffness-dependent progression in ovarian cancer

PMID: 30911294