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CNN3 Polyclonal antibody
CNN3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 11509-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HCT 116 cells, HAP1 cells, human kidney tissue, human skeletal muscle tissue, HEK-293 cells, HepG2 cells, NIH/3T3 cells |
Positive IP detected in | HEK-293 cells, U-87MG cells |
Positive IHC detected in | human prostate cancer tissue, human hysteromyoma tissue, human skin tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 2 publications below |
IHC | See 1 publications below |
IF | See 1 publications below |
Product Information
11509-1-AP targets CNN3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CNN3 fusion protein Ag2068 相同性解析による交差性が予測される生物種 |
Full Name | calponin 3, acidic |
Calculated molecular weight | 329 aa, 36 kDa |
Observed molecular weight | 36 kDa |
GenBank accession number | BC025372 |
Gene symbol | CNN3 |
Gene ID (NCBI) | 1266 |
RRID | AB_2082140 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for CNN3 antibody 11509-1-AP | Download protocol |
IHC protocol for CNN3 antibody 11509-1-AP | Download protocol |
IF protocol for CNN3 antibody 11509-1-AP | Download protocol |
IP protocol for CNN3 antibody 11509-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Rep Spatiotemporal dynamics of inner ear sensory and non-sensory cells revealed by single-cell transcriptomics. | ||
J Proteome Res Comparative Proteomics Reveals Prolonged Corneal Preservation Impaired Ocular Surface Immunity Accompanied by Fibrosis in Human Stroma | ||