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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CNNM3 Polyclonal antibody (13976-1-AP)

    WB analysis using 13976-1-AP (same clone as 13976-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13976-1-AP (CNNM3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13976-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of K-562 cells using CNNM3 Polyclonal antibody (13976-1-AP)

    IP experiment of K-562 using 13976-1-AP (same clone as 13976-1-PBS)

    IP result of anti-CNNM3 (IP:13976-1-AP, 4ug; Detection:13976-1-AP 1:500) with K-562 cells lysate 3600ug. This data was developed using the same antibody clone with 13976-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using CNNM3 Polyclonal antibody (13976-1-AP)

    IHC staining of human spleen using 13976-1-AP (same clone as 13976-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 13976-1-AP (CNNM3 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13976-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using CNNM3 Polyclonal antibody (13976-1-AP)

    IHC staining of human spleen using 13976-1-AP (same clone as 13976-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 13976-1-AP (CNNM3 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13976-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CNNM3 Polyclonal antibody (13976-1-AP)

    IF Staining of A431 using 13976-1-AP (same clone as 13976-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CNNM3 antibody (13976-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13976-1-PBS in a different storage buffer formulation.

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CNNM3 Polyclonal antibody, PBS Only

CNNM3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 13976-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13976-1-PBS targets CNNM3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CNNM3 fusion protein Ag5012 相同性解析による交差性が予測される生物種
Full Name cyclin M3
Calculated molecular weight 76 kDa
Observed molecular weight 85 kDa
GenBank accession numberBC037272
Gene Symbol CNNM3
Gene ID (NCBI) 26505
RRIDAB_2082153
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NE01
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Human Cyclin M 3 (CNNM3) has five transmembrane domains located at the N-terminal region and a C terminus containing a CBS pair domain and a cyclin box, both of which are part of the ACD region. CNNM3 magnesium transporter raises the intracellular magnesium content of cancer cells contributing to increased tumor growth and the acquisition of oncogenic properties (PMID: 24632616).