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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using CNNM4 Polyclonal antibody (14066-1-AP)

    WB analysis of HEK-293 using 14066-1-AP

    WB result of CNNM4 antibody (14066-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNNM4 transfected HEK-293 cells.

  • Western Blot (WB) analysis of various lysates using CNNM4 Polyclonal antibody (14066-1-AP)

    WB analysis using 14066-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14066-1-AP (CNNM4 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using CNNM4 Polyclonal antibody (14066-1-AP)

    IP experiment of mouse testis using 14066-1-AP

    IP result of anti-CNNM4 (IP:14066-1-AP, 4ug; Detection:14066-1-AP 1:1000) with mouse testis tissue lysate 4400ug.

  • Immunohistochemistry (IHC) staining of human colon tissue using CNNM4 Polyclonal antibody (14066-1-AP)

    IHC staining of human colon using 14066-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14066-1-AP (CNNM4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CNNM4 Polyclonal antibody (14066-1-AP)

    IF Staining of MCF-7 using 14066-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CNNM4 antibody (14066-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  • KD/KO Validated

CNNM4 Polyclonal antibody

CNNM4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 14066-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

KIAA1592, Cyclin-M4, cyclin M4, Ancient conserved domain-containing protein 4, ACDP4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, mouse colon tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14066-1-AP targets CNNM4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CNNM4 fusion protein Ag5121 相同性解析による交差性が予測される生物種
Full Name cyclin M4
Calculated molecular weight 87 kDa
Observed molecular weight 90-100 kDa
GenBank accession numberBC063295
Gene Symbol CNNM4
Gene ID (NCBI) 26504
RRIDAB_2878009
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6P4Q7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ancient conserved domain-containing protein 4/cyclin M4 (CNNM4), a membrane protein that stimulates Mg2+ efflux, binds phosphatase of regenerating liver (PRL), which is frequently overexpressed in malignant human cancers (PMID: 25347473). CNNM4 is predominant in the brain, bone marrow, immune system, and especially abundant in the intestinal tract. CNNM4 localizes at the basolateral membrane of the intestinal epithelia and mediates transcellular Mg2+ transport (PMID: 27006114).

Protocols

Product Specific Protocols
WB protocol for CNNM4 antibody 14066-1-APDownload protocol
IHC protocol for CNNM4 antibody 14066-1-APDownload protocol
IF protocol for CNNM4 antibody 14066-1-APDownload protocol
IP protocol for CNNM4 antibody 14066-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IHC

Nat Commun

Restoring cellular magnesium balance through Cyclin M4 protects against acetaminophen-induced liver damage

Authors - Irene González-Recio
View Article