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  • Featured Product
  • KD/KO Validated

CNOT1 Polyclonal antibody

CNOT1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, chIP, ELISA

Conjugate

Unconjugated

Cat no : 14276-1-AP

Synonyms

hNOT1, CDC39, CCR4-NOT transcription complex subunit 1, CCR4-associated factor 1, AD-005

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HeLa cells using CNOT1 Polyclonal antibody (14276-1-AP)

    WB analysis of HeLa using 14276-1-AP

    WB result of CNOT1 antibody (14276-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNOT1 transfected HeLa cells.

  • Western Blot (WB) analysis of human brain tissue using CNOT1 Polyclonal antibody (14276-1-AP)

    WB analysis of human brain using 14276-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 14276-1-AP (CNOT1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK293 cells using CNOT1 Polyclonal antibody (14276-1-AP)

    WB analysis of HEK293 cells using 14276-1-AP

    WB result from Dr. Vishram Kedar. Lysates from transfected HEK293 cells as indicated in the figure above, were subjected to the western bloting using rabbit CNOT1 antibody (Cat # 14276-1-AP) from Protein-Tech group, Inc, at dilution 1:1000 in 5% non-fat dry milk/TBST, followed by goat anti-rabbit HRP (Bio-RAD) at the dilution of 1:10,000 in 5% non-fat dry milk/TBST. Only bands of the size of 174 kDa were seen. 267 kDa bands of CNOT1 were not visible in this short exposure.

  • Western Blot (WB) analysis of mouse kidney tissue using CNOT1 Polyclonal antibody (14276-1-AP)

    WB analysis of mouse kidney using 14276-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14276-1-AP (CNOT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using CNOT1 Polyclonal antibody (14276-1-AP)

    WB analysis of mouse spleen using 14276-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14276-1-AP (CNOT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using CNOT1 Polyclonal antibody (14276-1-AP)

    WB analysis of mouse brain using 14276-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14276-1-AP (CNOT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using CNOT1 Polyclonal antibody (14276-1-AP)

    WB analysis of mouse heart using 14276-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 14276-1-AP (CNOT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using CNOT1 Polyclonal antibody (14276-1-AP)

    WB analysis of mouse thymus using 14276-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14276-1-AP (CNOT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using CNOT1 Polyclonal antibody (14276-1-AP)

    WB analysis of mouse kidney using 14276-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14276-1-AP (CNOT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CNOT1 Polyclonal antibody (14276-1-AP)

    WB analysis of HEK-293 using 14276-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14276-1-AP (CNOT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse kidney tissue using CNOT1 Polyclonal antibody (14276-1-AP)

    IP experiment of mouse kidney using 14276-1-AP

    IP result of anti-CNOT1 (IP:14276-1-AP, 5ug; Detection:14276-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

  • Immunoprecipitation (IP) experiment of HeLa cells using CNOT1 Polyclonal antibody (14276-1-AP)

    IP experiment of HeLa using 14276-1-AP

    IP result of anti-CNOT1 (IP:14276-1-AP, 4ug; Detection:14276-1-AP 1:600) with HeLa cells lysate 1085 ug.

  • Immunohistochemistry (IHC) staining of human heart tissue using CNOT1 Polyclonal antibody (14276-1-AP)

    IHC staining of human heart using 14276-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14276-1-AP (CNOT1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using CNOT1 Polyclonal antibody (14276-1-AP)

    IHC staining of human heart using 14276-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart slide using 14276-1-AP (CNOT1 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human spleen tissue using CNOT1 Polyclonal antibody (14276-1-AP)

    IHC staining of human spleen using 14276-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 14276-1-AP (CNOT1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using CNOT1 Polyclonal antibody (14276-1-AP)

    IHC staining of human spleen using 14276-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 14276-1-AP (CNOT1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CNOT1 Polyclonal antibody (14276-1-AP)

    IHC staining of human kidney using 14276-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 14276-1-AP (CNOT1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CNOT1 Polyclonal antibody (14276-1-AP)

    IHC staining of human kidney using 14276-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 14276-1-AP (CNOT1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CNOT1 Polyclonal antibody (14276-1-AP)

    IF Staining of HEK-293 using 14276-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CNOT1 antibody (14276-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CNOT1 Polyclonal antibody (14276-1-AP)

    IF Staining of HEK-293 using 14276-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CNOT1 antibody (14276-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inhuman brain tissue, HEK293 cells, mouse kidney tissue, mouse spleen tissue, mouse thymus tissue, HEK-293 cells, mouse brain tissue, mouse heart tissue, HeLa cells
Positive IP detected inmouse kidney tissue, HeLa cells
Positive IHC detected inhuman heart tissue, human spleen tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14276-1-AP targets CNOT1 in WB, IHC, IF/ICC, IP, CoIP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CNOT1 fusion protein Ag5623 相同性解析による交差性が予測される生物種
Full Name CCR4-NOT transcription complex, subunit 1
Calculated molecular weight 267 kDa
Observed molecular weight 240-250 kDa, 174 kDa
GenBank accession numberBC040523
Gene symbol CNOT1
Gene ID (NCBI) 23019
RRIDAB_10888627
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CNOT1 is a component of CCR4-NOT protein complex, which is a regulator of RNA polymerase II transcription, acts as a transcription repressor. CCR4-NOT complex could participate in transcription as well as mRNA degradation. It's highly expressed in brain, heart, thymus, but weak in skeletal muscle and colon. CNOT1 undergoes alternative splicing to produce four isoforms.This is a rabbit polyclonal antibody raised against part chain of C-terminal CNOT1 of human origin. CNOT1 exsits as many isoforms and molecular weight of isoforms are 267, 241 and 173 kDa.

Protocols

Product Specific Protocols
WB protocol for CNOT1 antibody 14276-1-APDownload protocol
IHC protocol for CNOT1 antibody 14276-1-APDownload protocol
IF protocol for CNOT1 antibody 14276-1-APDownload protocol
IP protocol for CNOT1 antibody 14276-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

DND1 maintains germline stem cells via recruitment of the CCR4-NOT complex to target mRNAs.

Authors - Masashi Yamaji
View Article
humanWB

Cell

Roquin promotes constitutive mRNA decay via a conserved class of stem-loop recognition motifs.

Authors - Leppek Kathrin K
View Article
mouseWB

Cell

The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity.

Authors - Deniz Simsek
View Article
humanWB

Nat Cancer

SULT1A1-dependent sulfonation of alkylators is a lineage-dependent vulnerability of liver cancers

Authors - Lei Shi
View Article
WB

Mol Cell

An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation

Authors - Stephanie Pei Tung Yiu
View Article
humanWB

Mol Cell

Mechanism of ribosome-associated mRNA degradation during tubulin autoregulation

Authors - Markus Höpfler
  • KD Validated
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Aditya (Verified Customer) (06-20-2024)

Exactly how I would expect an antibodty to behave. Excellent. There is a nonspecific band at 100KDa, but this is probably some variant of CNOT1, so cant fault this at all.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: hek293
FH

Roy (Verified Customer) (06-12-2024)

It does detect CNOT1 by WB from HeLa extracts ( 1/1000 dilution - Overnight incubation at 4°C). It works very well to Immunoprecipitate CNOT1 from both cytoplasmic and nuclear fractions of HeLa cells (4µg /IP)

  • Applications: Western Blot, Immunoprecipitation
  • Primary Antibody Dilution: 1/1000 (WB)
  • Cell Tissue Type: HeLa
FH

aditya (Verified Customer) (10-25-2022)

This is brilliant. We use this for cnot1 pull downs and then use a seperate cnot1 antibody to image on the western. Great for identifying interacting partners.

  • Applications: Western Blot, Protein purification
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Hek293
FH

Tobias (Verified Customer) (10-25-2022)

  • Applications: Western Blot, Immunoprecipitation
FH

Parisa (Verified Customer) (10-17-2022)

good quality, high sensitivity

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Hek293
FH

Will (Verified Customer) (01-31-2019)

good antibody, would use again

  • Applications: Western Blot,