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  • KD/KO Validated

CNOT2 Polyclonal antibody

CNOT2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 10313-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF/ICC, ELISA

HSPC131, FLJ26456, CDC36, CCR4-NOT transcription complex subunit 2, CCR4-associated factor 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inNIH/3T3 cells, HEK-293 cells
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10313-1-AP targets CNOT2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CNOT2 fusion protein Ag0347 相同性解析による交差性が予測される生物種
Full Name CCR4-NOT transcription complex, subunit 2
Calculated molecular weight 60 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC002597
Gene Symbol CNOT2
Gene ID (NCBI) 4848
RRIDAB_2245072
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NZN8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The Ccr4-Not complex is evolutionarily conserved and controls gene expression both at the level of transcription initiation and of mRNA degradation. And this complex can regulate pol II transcription. Promoter targeting of CCR4-NOT transcription complex subunit 2 (CNOT2) results in strong repression of pol II transcription. CNOT2-mediated repression is sensitive to the HDAC (histone deacetylase) inhibitor, TSA (trichostatin A).

Protocols

Product Specific Protocols
WB protocol for CNOT2 antibody 10313-1-APDownload protocol
IF protocol for CNOT2 antibody 10313-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Stem Cells

Cnot1, Cnot2, and Cnot3 maintain mouse and human ESC identity and inhibit extraembryonic differentiation.

Authors - Zheng Xiaofeng X
  • KD Validated
mouseWB

Stem Cell Reports

CNOT3-Dependent mRNA Deadenylation Safeguards the Pluripotent State.

Authors - Xiaofeng Zheng
mouseWB

RNA Biol

Essential functions of the CNOT7/8 catalytic subunits of the CCR4-NOT complex in mRNA regulation and cell viability.

Authors - Dina Mostafa
mouseWB

Stem Cells Dev

Identification of Ccr4-Not complex components as regulators of transition from partial to genuine induced pluripotent stem cells.

Authors - Kamon Masayoshi M
  • KD Validated
mouseWB

bioRxiv

Cnot3 is required for male germ cell development and spermatogonial stem cell maintenance

Authors - Qing Chen
humanWB

Methods Mol Biol

Dissecting the Role of the Ccr4-Not Deadenylase Complex in Pluripotency and Differentiation

Authors - Xiukun Wang
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