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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CNOT4 Monoclonal antibody

CNOT4 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

4H5H3

Cat no : 67798-1-Ig

Synonyms

E3 ubiquitin-protein ligase CNOT4, CLONE243, CCR4-NOT transcription complex subunit 4, CCR4-associated factor 4, CCR4 associated factor 4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CNOT4 Monoclonal antibody (67798-1-Ig)

    WB analysis using 67798-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67798-1-Ig (CNOT4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • Western Blot (WB) analysis of MCF-7 cells using CNOT4 Monoclonal antibody (67798-1-Ig)

    WB analysis of MCF-7 using 67798-1-Ig

    WB result of CNOT4 antibody (67798-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNOT4 transfected MCF-7 cells.

  • Western Blot (WB) analysis of U2OS cells using CNOT4 Monoclonal antibody (67798-1-Ig)

    WB analysis of U2OS using 67798-1-Ig

    U2OS cells were subjected to SDS PAGE followed by western blot with 67798-1-Ig (CNOT4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CNOT4 Monoclonal antibody (67798-1-Ig)

    IHC staining of human colon cancer using 67798-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67798-1-Ig (CNOT4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CNOT4 Monoclonal antibody (67798-1-Ig)

    IHC staining of human colon cancer using 67798-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67798-1-Ig (CNOT4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using CNOT4 Monoclonal antibody (67798-1-Ig)

    IF Staining of human colon cancer using 67798-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CNOT4 antibody (67798-1-Ig, Clone: 4H5H3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using CNOT4 Monoclonal antibody (67798-1-Ig)

    IF Staining of human colon cancer using 67798-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CNOT4 antibody (67798-1-Ig, Clone: 4H5H3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CNOT4 Monoclonal antibody (67798-1-Ig)

    IF Staining of HepG2 using 67798-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CNOT4 antibody (67798-1-Ig, Clone: 4H5H3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, U2OS cells, MCF-7 cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells.
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman colon cancer tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67798-1-Ig targets CNOT4 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CNOT4 fusion protein Ag29964 相同性解析による交差性が予測される生物種
Full Name CCR4-NOT transcription complex, subunit 4
Calculated molecular weight 575 aa, 64 kDa
Observed molecular weight 64-85 kDa
GenBank accession numberBC035590
Gene symbol CNOT4
Gene ID (NCBI) 4850
RRIDAB_2918562
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CNOT4, also known as CCR4-NOT transcription complex subunit 4 , has E3 ubiquitin ligase activity, and promotes ubiquitination and degradation of target proteins. CNOT4 is involved in activation of the JAK/STAT pathway. CNOT4 exist many isoforms and the range of its molecular weight from 48 to 84 kDa.

Protocols

Product Specific Protocols
WB protocol for CNOT4 antibody 67798-1-IgDownload protocol
IHC protocol for CNOT4 antibody 67798-1-IgDownload protocol
IF protocol for CNOT4 antibody 67798-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Aditya (Verified Customer) (10-08-2024)

excellent

  • Applications: Western Blot