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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CNOT4 Monoclonal antibody

CNOT4 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

4H5H3

Cat no : 67798-1-Ig

Synonyms

E3 ubiquitin-protein ligase CNOT4, CLONE243, CCR4-NOT transcription complex subunit 4, CCR4-associated factor 4, CCR4 associated factor 4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CNOT4 Monoclonal antibody (67798-1-Ig)

    WB analysis using 67798-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67798-1-Ig (CNOT4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • Western Blot (WB) analysis of MCF-7 cells using CNOT4 Monoclonal antibody (67798-1-Ig)

    WB analysis of MCF-7 using 67798-1-Ig

    WB result of CNOT4 antibody (67798-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNOT4 transfected MCF-7 cells.

  • Western Blot (WB) analysis of U2OS cells using CNOT4 Monoclonal antibody (67798-1-Ig)

    WB analysis of U2OS using 67798-1-Ig

    U2OS cells were subjected to SDS PAGE followed by western blot with 67798-1-Ig (CNOT4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CNOT4 Monoclonal antibody (67798-1-Ig)

    IHC staining of human colon cancer using 67798-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67798-1-Ig (CNOT4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CNOT4 Monoclonal antibody (67798-1-Ig)

    IHC staining of human colon cancer using 67798-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67798-1-Ig (CNOT4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using CNOT4 Monoclonal antibody (67798-1-Ig)

    IF Staining of human colon cancer using 67798-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CNOT4 antibody (67798-1-Ig, Clone: 4H5H3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using CNOT4 Monoclonal antibody (67798-1-Ig)

    IF Staining of human colon cancer using 67798-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CNOT4 antibody (67798-1-Ig, Clone: 4H5H3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CNOT4 Monoclonal antibody (67798-1-Ig)

    IF Staining of HepG2 using 67798-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CNOT4 antibody (67798-1-Ig, Clone: 4H5H3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, U2OS cells, MCF-7 cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells.
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman colon cancer tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67798-1-Ig targets CNOT4 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CNOT4 fusion protein Ag29964 相同性解析による交差性が予測される生物種
Full Name CCR4-NOT transcription complex, subunit 4
Calculated molecular weight 575 aa, 64 kDa
Observed molecular weight 64-85 kDa
GenBank accession numberBC035590
Gene symbol CNOT4
Gene ID (NCBI) 4850
RRIDAB_2918562
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CNOT4, also known as CCR4-NOT transcription complex subunit 4 , has E3 ubiquitin ligase activity, and promotes ubiquitination and degradation of target proteins. CNOT4 is involved in activation of the JAK/STAT pathway. CNOT4 exist many isoforms and the range of its molecular weight from 48 to 84 kDa.

Protocols

Product Specific Protocols
WB protocol for CNOT4 antibody 67798-1-IgDownload protocol
IHC protocol for CNOT4 antibody 67798-1-IgDownload protocol
IF protocol for CNOT4 antibody 67798-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols