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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CNOT4 Recombinant antibody (83615-4-RR)

    WB analysis using 83615-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83615-4-RR (CNOT4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using CNOT4 Recombinant antibody (83615-4-RR)

    IHC staining of human ovary cancer using 83615-4-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83615-4-RR (CNOT4 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CNOT4 Recombinant antibody (83615-4-RR)

    IF Staining of HeLa using 83615-4-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CNOT4 antibody (83615-4-RR, Clone: 240559E1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HeLa cells using CNOT4 Recombinant antibody (83615-4-RR)

    FC experiment of HeLa using 83615-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Cnot4 Recombinant Antibody (83615-4-RR, Clone:240559E1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83615-4-RR

    Affinity and Kinetic Characterization of 83615-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83615-4-RR against Human CNOT4 were performed. The affinity constant is 0.485 nM.

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CNOT4 Recombinant antibody

CNOT4 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83615-4-RR
Clone No.240559E1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

E3 ubiquitin-protein ligase CNOT4, CLONE243, CCR4-NOT transcription complex subunit 4, CCR4-associated factor 4, CCR4 associated factor 4

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, HEK-293T cells, HeLa cells, K-562 cells, Jurkat cells, NIH/3T3 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83615-4-RR targets CNOT4 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CNOT4 fusion protein Ag3163 相同性解析による交差性が予測される生物種
Full Name CCR4-NOT transcription complex, subunit 4
Calculated molecular weight 575 aa, 64 kDa
Observed molecular weight 64-85 kDa
GenBank accession numberBC035590
Gene Symbol CNOT4
Gene ID (NCBI) 4850
RRIDAB_3671229
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO95628
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CNOT4, also known as CCR4-NOT transcription complex subunit 4 , has E3 ubiquitin ligase activity, and promotes ubiquitination and degradation of target proteins. CNOT4 is involved in activation of the JAK/STAT pathway. CNOT4 exist many isoforms and the range of its molecular weight from 48 to 84 kDa.

Protocols

Product Specific Protocols
WB protocol for CNOT4 antibody 83615-4-RRDownload protocol
IHC protocol for CNOT4 antibody 83615-4-RRDownload protocol
IF protocol for CNOT4 antibody 83615-4-RRDownload protocol
FC protocol for CNOT4 antibody 83615-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols