• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

CNOT8 Polyclonal antibody

CNOT8 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 10752-1-AP

Synonyms

CALIF, CAF2, CAF1-like protein, Caf1b, CAF1 like protein

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of Jurkat cells using CNOT8 Polyclonal antibody (10752-1-AP)

    WB analysis of Jurkat using 10752-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10752-1-AP (CNOT8 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using CNOT8 Polyclonal antibody (10752-1-AP)

    WB analysis of mouse testis using 10752-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 10752-1-AP (CNOT8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using CNOT8 Polyclonal antibody (10752-1-AP)

    WB analysis of mouse testis using 10752-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 10752-1-AP (CNOT8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CNOT8 Polyclonal antibody (10752-1-AP)

    WB analysis of HeLa using 10752-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10752-1-AP (CNOT8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using CNOT8 Polyclonal antibody (10752-1-AP)

    IP experiment of mouse testis using 10752-1-AP

    IP result of anti-CNOT8 (IP:10752-1-AP, 4ug; Detection:10752-1-AP 1:600) with mouse testis tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CNOT8 Polyclonal antibody (10752-1-AP)

    IHC staining of human prostate cancer using 10752-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10752-1-AP (CNOT8 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CNOT8 Polyclonal antibody (10752-1-AP)

    IHC staining of human kidney using 10752-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10752-1-AP (CNOT8 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CNOT8 Polyclonal antibody (10752-1-AP)

    IHC staining of human kidney using 10752-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10752-1-AP (CNOT8 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CNOT8 Polyclonal antibody (10752-1-AP)

    IHC staining of human prostate cancer using 10752-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10752-1-AP (CNOT8 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CNOT8 Polyclonal antibody (10752-1-AP)

    IHC staining of human prostate cancer using 10752-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10752-1-AP (CNOT8 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CNOT8 Polyclonal antibody (10752-1-AP)

    IF Staining of HeLa using 10752-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 10752-1-AP (CNOT8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Positive WB detected inJurkat cells, HeLa cells, mouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman prostate cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10752-1-AP targets CNOT8 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CNOT8 fusion protein Ag1201 相同性解析による交差性が予測される生物種
Full Name CCR4-NOT transcription complex, subunit 8
Calculated molecular weight 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC017366
Gene symbol CNOT8
Gene ID (NCBI) 9337
RRIDAB_2082470
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CCR4-NOT transcription complex subunit 8(CNOT8), is a ubiquitous transcription factor, which required for a diverse set of processes. The CCR4-NOT complex is a global regulator of RNA polymerase II, and functions as general transcription regulation complex. It consisted part by CCR4, NOT1 to NOT5, and CAF1. This is a rabbit polyclonal antibody raised against the full-length chain of human CNOT8.

Protocols

Product Specific Protocols
WB protocol for CNOT8 antibody 10752-1-APDownload protocol
IHC protocol for CNOT8 antibody 10752-1-APDownload protocol
IF protocol for CNOT8 antibody 10752-1-APDownload protocol
IP protocol for CNOT8 antibody 10752-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

Mechanism of ribosome-associated mRNA degradation during tubulin autoregulation

Authors - Markus Höpfler
  • KD Validated
View Article
humanWB

Mol Biosyst

Large-scale investigation of oxygen response mutants in Saccharomyces cerevisiae.

Authors - Samanfar Bahram B
  • KD Validated
View Article
mouseWB

Cells

Translation Efficiency and Degradation of ER-Associated mRNAs Modulated by ER-Anchored poly(A)-Specific Ribonuclease (PARN).

Authors - Tian-Li Duan
View Article
humanWB

Nucleic Acids Res

BESST: a novel LncRNA knockout strategy with less genome perturbance

Authors - Shikuan Zhang
View Article
humanWB,IF

Cells

Disruption of the Mammalian Ccr4-Not Complex Contributes to Transcription-Mediated Genome Instability

Authors - Nafiseh Chalabi Hagkarim
  • KD Validated
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Aditya (Verified Customer) (10-08-2024)

works okay, better than cnot9

  • Applications: Western Blot
FH

Roy (Verified Customer) (06-12-2024)

Works well to detect CNOT8 by Western Blot (1/500 Dilution)

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/500
  • Cell Tissue Type: HeLa