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  • Featured Product
  • KD/KO Validated

CNOT9 Polyclonal antibody, PBS Only

CNOT9 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 22503-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using CNOT9 Polyclonal antibody (22503-1-AP)

    WB analysis of HepG2 using 22503-1-AP (same clone as 22503-1-PBS)

    WB result of RQCD1 antibody (22503-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RQCD1 transfected HepG2 cells. This data was developed using the same antibody clone with 22503-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CNOT9 Polyclonal antibody (22503-1-AP)

    WB analysis using 22503-1-AP (same clone as 22503-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22503-1-AP (CNOT9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22503-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse thymus tissue using CNOT9 Polyclonal antibody (22503-1-AP)

    WB analysis of mouse thymus using 22503-1-AP (same clone as 22503-1-PBS)

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 22503-1-AP (RQCD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22503-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using CNOT9 Polyclonal antibody (22503-1-AP)

    IP experiment of MCF-7 using 22503-1-AP (same clone as 22503-1-PBS)

    IP result of anti-CNOT9 (IP:22503-1-AP, 4ug; Detection:22503-1-AP 1:1000) with MCF-7 cells lysate 1280ug. This data was developed using the same antibody clone with 22503-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using CNOT9 Polyclonal antibody (22503-1-AP)

    IHC staining of human stomach cancer using 22503-1-AP (same clone as 22503-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 22503-1-AP (CNOT9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22503-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using CNOT9 Polyclonal antibody (22503-1-AP)

    IHC staining of human stomach cancer using 22503-1-AP (same clone as 22503-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 22503-1-AP (CNOT9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22503-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat colon tissue using CNOT9 Polyclonal antibody (22503-1-AP)

    IHC staining of rat colon using 22503-1-AP (same clone as 22503-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 22503-1-AP (CNOT9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22503-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat colon tissue using CNOT9 Polyclonal antibody (22503-1-AP)

    IHC staining of rat colon using 22503-1-AP (same clone as 22503-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 22503-1-AP (CNOT9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22503-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CNOT9 Polyclonal antibody (22503-1-AP)

    IF Staining of HepG2 using 22503-1-AP (same clone as 22503-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CNOT9 antibody (22503-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 22503-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using CNOT9 Polyclonal antibody (22503-1-AP)

    IF Staining of hTERT-RPE1 using 22503-1-AP (same clone as 22503-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CNOT9 antibody (22503-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 22503-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22503-1-PBS targets CNOT9 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CNOT9 fusion protein Ag18197 相同性解析による交差性が予測される生物種
Full Name RCD1 required for cell differentiation1 homolog (S. pombe)
Calculated molecular weight 299 aa, 34 kDa
Observed molecular weight 30-40 kDa
GenBank accession numberBC007102
Gene symbol CNOT9
Gene ID (NCBI) 9125
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RQCD1, also known as CNOT9, is a highly conserved transcriptional cofactor predominantly expressed in actively diffierentiating tissues like testis and thymus. It is a component of the CCR4-NOT complex, the major effector complex in miRNA-mediated gene silencing. Overexpression of RQCD1 has been reported in serveral tumors, indicating that it may be a potential molecular target for cancer treatment.