CNPase Polyclonal antibody
CNPase Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF-P, FC (Intra), ELISA
Conjugate
Unconjugated
Cat no : 13427-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 7 publications below |
| IHC | See 8 publications below |
| IF | See 4 publications below |
Product Information
13427-1-AP targets CNPase in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CNPase fusion protein Ag4227 相同性解析による交差性が予測される生物種 |
| Full Name | 2',3'-cyclic nucleotide 3' phosphodiesterase |
| Calculated molecular weight | 421 aa, 48 kDa |
| Observed molecular weight | 45-48 kDa |
| GenBank accession number | BC028040 |
| Gene symbol | CNPase |
| Gene ID (NCBI) | 1267 |
| RRID | AB_2082477 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CNP(2',3'-cyclic-nucleotide 3'-phosphodiesterase) belongs to the cyclic nucleotide phosphodiesterase family. It plays an essential role in axonal survival but not in myelin assembly, coupling oligodendroglial functions in axonal support and myelination. This protein has 2 isoforms produced by alternative splicing with molecular mass of 45 and 48 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CNPase antibody 13427-1-AP | Download protocol |
| IHC protocol for CNPase antibody 13427-1-AP | Download protocol |
| IF protocol for CNPase antibody 13427-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Neurosci TREM2 interacts with TDP-43 and mediates microglial neuroprotection against TDP-43-related neurodegeneration. | ||
J Clin Invest Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling. | ||
J Pineal Res Circadian misalignment impairs oligodendrocyte myelination via Bmal1 overexpression leading to anxiety and depression-like behaviors | ||
Acta Physiol (Oxf) Keratin 1 attenuates hypoxic pulmonary artery hypertension by suppressing pulmonary artery media smooth muscle expansion. | ||
Mol Nutr Food Res A High MCT-Based Ketogenic Diet Suppresses Th1 and Th17 Responses to Ameliorate Experimental Autoimmune Encephalomyelitis in Mice by Inhibiting GSDMD and JAK2-STAT3/4 Pathways | ||
Front Cell Neurosci Neural Stem Cells Overexpressing Nerve Growth Factor Improve Functional Recovery in Rats Following Spinal Cord Injury via Modulating Microenvironment and Enhancing Endogenous Neurogenesis. |
