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CNPY2, MSAP Monoclonal antibody

CNPY2, MSAP Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1G8G11

Cat no : 66173-1-Ig

Synonyms

canopy 2 homolog (zebrafish), CNPY2, CNPY2, MSAP, CNPY2,MSAP, HP10390, MSAP, Protein canopy homolog 2, TMEM4, Transmembrane protein 4, ZSIG9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CNPY2, MSAP Monoclonal antibody (66173-1-Ig)

    WB analysis using 66173-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66173-1-Ig (CNPY2, MSAP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of HepG2 cells using CNPY2, MSAP Monoclonal antibody (66173-1-Ig)

    WB analysis of HepG2 using 66173-1-Ig

    WB result of CNPY2, MSAP antibody (66173-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNPY2, MSAP transfected HepG2 cells.

  • Western Blot (WB) analysis of rat liver tissue using CNPY2, MSAP Monoclonal antibody (66173-1-Ig)

    WB analysis of rat liver using 66173-1-Ig

    rat liver tissue were subjected to SDS PAGE followed by western blot with 66173-1-Ig (CNPY2, MSAP antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using CNPY2, MSAP Monoclonal antibody (66173-1-Ig)

    WB analysis of human placenta using 66173-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66173-1-Ig (CNPY2, MSAP antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using CNPY2, MSAP Monoclonal antibody (66173-1-Ig)

    WB analysis of HepG2 using 66173-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66173-1-Ig (CNPY2, MSAP antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using CNPY2, MSAP Monoclonal antibody (66173-1-Ig)

    IHC staining of human pancreas cancer using 66173-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66173-1-Ig (CNPY2, MSAP antibody at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using CNPY2, MSAP Monoclonal antibody (66173-1-Ig)

    IHC staining of human pancreas cancer using 66173-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66173-1-Ig (CNPY2, MSAP antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CNPY2, MSAP Monoclonal antibody (66173-1-Ig)

    IF Staining of HepG2 using 66173-1-Ig

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66173-1-Ig(CNPY2, MSAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-mouse IgG(H+L).

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Tested Applications

Positive WB detected inA549 cells, HepG2 cells, rat liver tissue, human placenta tissue, pig liver tissue, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells, NIH/3T3 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66173-1-Ig targets CNPY2, MSAP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CNPY2, MSAP fusion protein Ag6363 相同性解析による交差性が予測される生物種
Full Name canopy 2 homolog (zebrafish)
Calculated molecular weight 21 kDa
Observed molecular weight 18-21 kDa
GenBank accession numberBC065015
Gene symbol CNPY2
Gene ID (NCBI) 10330
RRIDAB_2881568
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CNPY2, also named as MSAP, TMEM4 and ZSIG9, is a positive regulator of neurite outgrowth. It interacts with the ezrin-radixin-moesin (ERM)-like myosin regulatory light chain-interacting protein (MIR), and the two proteins are co-localized in cell lines and in primary neurons. CNPY2 can stabilize MRLC and thus bring about an increase in neurite outgrowth. (PMID: 12826659 ) This antibody is specific to CNPY2

Protocols

Product Specific Protocols
WB protocol for CNPY2, MSAP antibody 66173-1-IgDownload protocol
IHC protocol for CNPY2, MSAP antibody 66173-1-IgDownload protocol
IF protocol for CNPY2, MSAP antibody 66173-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols