Featured Product
KD/KO Validated

CNPY3 Polyclonal antibody

Name: CNPY3 Polyclonal antibody
Brand: Proteintech
SKU: 15215-1-AP

CNPY3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 15215-1-AP

Synonyms

Expanded repeat-domain protein CAG/CTG 5, ERDA5, CTG4A, CTG repeat protein 4a, canopy 3 homolog (zebrafish)

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of human brain using 15215-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 15215-1-AP (CNPY3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 15215-1-AP
WB result of CNPY3 antibody (15215-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNPY3 transfected A549 cells.
WB analysis using 15215-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15215-1-AP (CNPY3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 15215-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 15215-1-AP (CNPY3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse kidney using 15215-1-AP
IP result of anti-CNPY3 (IP:15215-1-AP, 3ug; Detection:15215-1-AP 1:500) with mouse kidney tissue lysate 6000ug.
IHC staining of human colon cancer using 15215-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15215-1-AP (CNPY3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 15215-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15215-1-AP (CNPY3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HEK-293 using 15215-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CNPY3 antibody (15215-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

-/ -

Cat No. 15215-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, Jurkat cells, human brain tissue, A549 cells
Positive IP detected in	mouse kidney tissue
Positive IHC detected in	human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
IHC	See 2 publications below

Product Information

15215-1-AP targets CNPY3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CNPY3 fusion protein Ag7382 相同性解析による交差性が予測される生物種
Full Name	canopy 3 homolog (zebrafish)
Calculated molecular weight	31 kDa
Observed molecular weight	30-40 kDa
GenBank accession number	BC004423
Gene symbol	CNPY3
Gene ID (NCBI)	10695
RRID	AB_11182172
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for CNPY3 antibody 15215-1-AP	Download protocol
IHC protocol for CNPY3 antibody 15215-1-AP	Download protocol
IF protocol for CNPY3 antibody 15215-1-AP	Download protocol
IP protocol for CNPY3 antibody 15215-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC	Cell Oncol (Dordr) The SLITRK4-CNPY3 axis promotes liver metastasis of gastric cancer by enhancing the endocytosis and recycling of TrkB in tumour cells Authors - Yao-Qi Zhou View Article
human	WB	J Neuroimmunol A novel rare variant of CNPY3 from familial NMOSD impairs the TLR-mediated immune response Authors - Yongxin Mo View Article
human	WB,IHC	World J Gastrointest Oncol Canopy FGF signaling regulator 3 affects prognosis, immune infiltration, and PI3K/AKT pathway in colon adenocarcinoma Authors - Xu-Can Gao View Article

製品

アプリケーション

cGMPグレード製品

サポート

カスタム対応

プロモーション

企業情報

抗体

免疫アッセイ

サイトカイン・増殖因子

ChromoTek VHH（Nanobody®）試薬

サポートツール・試薬

ゲノミクス試薬

研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CNPY3 Polyclonal antibody

CNPY3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of human brain using 15215-1-AP

WB analysis of A549 using 15215-1-AP

WB analysis using 15215-1-AP

WB analysis of Jurkat using 15215-1-AP

IP experiment of mouse kidney using 15215-1-AP

IHC staining of human colon cancer using 15215-1-AP

IHC staining of human colon cancer using 15215-1-AP

IF Staining of HEK-293 using 15215-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

The SLITRK4-CNPY3 axis promotes liver metastasis of gastric cancer by enhancing the endocytosis and recycling of TrkB in tumour cells

A novel rare variant of CNPY3 from familial NMOSD impairs the TLR-mediated immune response

Canopy FGF signaling regulator 3 affects prognosis, immune infiltration, and PI3K/AKT pathway in colon adenocarcinoma