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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CNPY4 Polyclonal antibody

CNPY4 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 22086-1-AP

Synonyms

UNQ1909/PRO4354, PSEC0237, Protein canopy homolog 4, PRAT4B, canopy 4 homolog (zebrafish)

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CNPY4 Polyclonal antibody (22086-1-AP)

    WB analysis of HeLa using 22086-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 22086-1-AP (CNPY4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CNPY4 Polyclonal antibody (22086-1-AP)

    WB analysis of HeLa using 22086-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 22086-1-AP (CNPY4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CNPY4 Polyclonal antibody (22086-1-AP)

    IF Staining of HeLa using 22086-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 22086-1-AP (CNPY4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22086-1-AP targets CNPY4 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CNPY4 fusion protein Ag17285 相同性解析による交差性が予測される生物種
Full Name canopy 4 homolog (zebrafish)
Calculated molecular weight 248 aa, 28 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC032339
Gene symbol CNPY4
Gene ID (NCBI) 245812
RRIDAB_2878990
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CNPY4 antibody 22086-1-APDownload protocol
IF protocol for CNPY4 antibody 22086-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols