Validation Data Gallery
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Tested Applications
| Positive WB detected in | SH-SY5Y cells, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 19 publications below |
| IHC | See 5 publications below |
| IF | See 4 publications below |
Product Information
17978-1-AP targets Cannabinoid receptor 1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Cannabinoid receptor 1 fusion protein Ag12371 相同性解析による交差性が予測される生物種 |
| Full Name | cannabinoid receptor 1 (brain) |
| Calculated molecular weight | 472 aa, 53 kDa |
| Observed molecular weight | 45 kDa, 66 kDa |
| GenBank accession number | BC074812 |
| Gene Symbol | Cannabinoid receptor 1 |
| Gene ID (NCBI) | 1268 |
| RRID | AB_10859098 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P21554 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cannabinoid receptor 1 (CNR1, or CB1) and CNR2 (CB2) are members of the guanine-nucleotide-binding protein (G-protein) coupled receptor family. The CB1 receptor is expressed mainly in the brain. The CB2 receptor is expressed mainly in the immune system and in hematopoietic cells. The two receptors have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Cannabinoid receptor 1 antibody 17978-1-AP | Download protocol |
| IHC protocol for Cannabinoid receptor 1 antibody 17978-1-AP | Download protocol |
| IF protocol for Cannabinoid receptor 1 antibody 17978-1-AP | Download protocol |
| FC protocol for Cannabinoid receptor 1 antibody 17978-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Elife p21-activated kinase 1 restricts tonic endocannabinoid signaling in the hippocampus. | ||
Chin Med Electroacupuncture repairs intestinal barrier by upregulating CB1 through gut microbiota in DSS-induced acute colitis | ||
Hum Mol Genet BDNF overexpression prevents cognitive deficit elicited by adolescent cannabis exposure and host susceptibility interaction. | ||
Phytomedicine Endocannabinoid signalling/cannabinoid receptor 2 is involved in icariin-mediated protective effects against bleomycin-induced pulmonary fibrosis. | ||
Food Funct L-Theanine regulates glutamine metabolism and immune function by binding to cannabinoid receptor 1. | ||
Int J Cardiol Indoxyl sulfate upregulates the cannabinoid type 1 receptor gene via an ATF3/c-Jun complex-mediated signaling pathway in the model of uremic cardiomyopathy. |