Validation Data Gallery

Filter: AllWBIHC

WB analysis using 10796-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10796-1-AP (CNTFR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 10796-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10796-1-AP (CNTFR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 10796-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 10796-1-AP (CNTFR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse cerebellum using 10796-1-AP
mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 10796-1-AP (CNTFR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of mouse brain using 10796-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10796-1-AP (CNTFR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 10796-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10796-1-AP (CNTFR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human heart using 10796-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10796-1-AP (CNTFR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human heart using 10796-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10796-1-AP (CNTFR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CNTFR Polyclonal antibody

CNTFR Polyclonal Antibody for WB, IHC, ELISA

Cat No. 10796-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

CNTF receptor subunit alpha, CNTFR, CNTFR alpha

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse brain tissue, human brain tissue, mouse cerebellum tissue, SH-SY5Y cells, rat brain tissue
Positive IHC detected in	mouse brain tissue, human heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below

Product Information

10796-1-AP targets CNTFR in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CNTFR fusion protein Ag1189 相同性解析による交差性が予測される生物種
Full Name	ciliary neurotrophic factor receptor
Calculated molecular weight	41 kDa
Observed molecular weight	45-50 kDa
GenBank accession number	BC001492
Gene Symbol	CNTFR
Gene ID (NCBI)	1271
RRID	AB_2083194
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P26992
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Ciliary neurotrophic factor (CNTF) is a pluripotent neurocytokine. The functional receptor complex of CNTF is composed of the CNTF receptor alpha (CNTFR), gp130 and the leukemia inhibitory factor receptor (LIFR) (PMID: 11943154). Binding of CNTF to CNTFR recruits gp130 and LIFR, which leads to activation of JAK/STAT and Ras/Raf/MAPK pathways.

Protocols

Product Specific Protocols
WB protocol for CNTFR antibody 10796-1-AP	Download protocol
IHC protocol for CNTFR antibody 10796-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	WB	Front Pharmacol Synergic Neuroprotection Between Ligusticum Chuanxiong Hort and Borneol Against Ischemic Stroke by Neurogenesis via Modulating Reactive Astrogliosis and Maintaining the Blood-Brain Barrier. Authors - Bin Yu View Article
human	WB	bioRxiv Patient-derived Induced Pluripotent Stem Cells as a Model to Study Frontotemporal Dementia Pathologies Authors - Sonia Infante-Tadeo View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 10796-1-AP

WB analysis of SH-SY5Y using 10796-1-AP

WB analysis of human brain using 10796-1-AP

WB analysis of mouse cerebellum using 10796-1-AP

IHC staining of mouse brain using 10796-1-AP