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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CNTFR Polyclonal antibody

CNTFR Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10796-1-AP

Synonyms

CNTF receptor subunit alpha, CNTFR, CNTFR alpha

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CNTFR Polyclonal antibody (10796-1-AP)

    WB analysis using 10796-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10796-1-AP (CNTFR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using CNTFR Polyclonal antibody (10796-1-AP)

    WB analysis of human brain using 10796-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 10796-1-AP (CNTFR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse cerebellum tissue using CNTFR Polyclonal antibody (10796-1-AP)

    WB analysis of mouse cerebellum using 10796-1-AP

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 10796-1-AP (CNTFR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using CNTFR Polyclonal antibody (10796-1-AP)

    WB analysis of SH-SY5Y using 10796-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10796-1-AP (CNTFR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CNTFR Polyclonal antibody (10796-1-AP)

    IHC staining of mouse brain using 10796-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10796-1-AP (CNTFR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CNTFR Polyclonal antibody (10796-1-AP)

    IHC staining of mouse brain using 10796-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10796-1-AP (CNTFR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human heart tissue using CNTFR Polyclonal antibody (10796-1-AP)

    IHC staining of human heart using 10796-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10796-1-AP (CNTFR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human heart tissue using CNTFR Polyclonal antibody (10796-1-AP)

    IHC staining of human heart using 10796-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10796-1-AP (CNTFR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue, mouse cerebellum tissue, SH-SY5Y cells, rat brain tissue
Positive IHC detected inmouse brain tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

10796-1-AP targets CNTFR in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CNTFR fusion protein Ag1189 相同性解析による交差性が予測される生物種
Full Name ciliary neurotrophic factor receptor
Calculated molecular weight 41 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC001492
Gene symbol CNTFR
Gene ID (NCBI) 1271
RRIDAB_2083194
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ciliary neurotrophic factor (CNTF) is a pluripotent neurocytokine. The functional receptor complex of CNTF is composed of the CNTF receptor alpha (CNTFR), gp130 and the leukemia inhibitory factor receptor (LIFR) (PMID: 11943154). Binding of CNTF to CNTFR recruits gp130 and LIFR, which leads to activation of JAK/STAT and Ras/Raf/MAPK pathways.

Protocols

Product Specific Protocols
WB protocol for CNTFR antibody 10796-1-APDownload protocol
IHC protocol for CNTFR antibody 10796-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Front Pharmacol

Synergic Neuroprotection Between Ligusticum Chuanxiong Hort and Borneol Against Ischemic Stroke by Neurogenesis via Modulating Reactive Astrogliosis and Maintaining the Blood-Brain Barrier.

Authors - Bin Yu
View Article