• Featured Product
  • KD/KO Validated

CNTN1 Polyclonal antibody

CNTN1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13843-1-AP

Synonyms

CNTN1, contactin 1, F3, Glycoprotein gp135, GP135, Neural cell surface protein F3


☆対象製品が30%オフ!組換えタンパク質&免染用試薬 >>> キャンペーン実施中!


Tested Applications

Positive WB detected inmouse brain tissue, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue
Positive IP detected inrat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13843-1-AP targets CNTN1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CNTN1 fusion protein Ag4803 相同性解析による交差性が予測される生物種
Full Name contactin 1
Calculated molecular weight 1018 aa, 113 kDa
Observed molecular weight 135 kDa
GenBank accession numberBC036569
Gene symbol CNTN1
Gene ID (NCBI) 1272
RRIDAB_2877981
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Contactin-1 (CNTN1), also known as Glycoprotein gp135 or Neural cell surface protein F3, is a neuronal cell adhesion molecule of the immunoglobulin superfamily (PMID: 3049624). It consists of six Ig domains, four fibronectin-like motifs, and a glycosylphosphatidylinositol (GPI)-moiety. Expressed by the central and peripheral neurons, CNTN1 contributes to the formation and function of neuronal connections. It plays a role in axon extension and formation of septate-like junctions between axons and myelinating glial cells (PMID: 23724143). In addition, acting as a ligand for Notch, CNTN1 initiates a NOTCH/DTX1 signaling pathway that promotes oligodendrocyte maturation and myelination (PMID: 14567914).

Protocols

Product Specific Protocols
WB protocol for CNTN1 antibody 13843-1-APDownload protocol
IHC protocol for CNTN1 antibody 13843-1-APDownload protocol
IP protocol for CNTN1 antibody 13843-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Commun

Identification of genes associated with cortical malformation using a transposon-mediated somatic mutagenesis screen in mice.

Authors - I-Ling Lu
  • KD Validated
ratWB

Nat Commun

Spatial mapping of juxtacrine axo-glial interactions identifies novel molecules in peripheral myelination.

Authors - Y Poitelon
humanIHC

Ann Clin Transl Neurol

CIDP/autoimmune nodopathies with nephropathy: a case series study

Authors - Yuwei Tang
mouseIF,WB

Front Mol Neurosci

Contactin-1/F3 Regulates Neuronal Migration and Morphogenesis Through Modulating RhoA Activity.

Authors - Yi-An Chen
  • KD Validated
humanWB

Front Genet

CNTN-1 Upregulation Induced by Low-Dose Cisplatin Promotes Malignant Progression of Lung Adenocarcinoma Cells via Activation of Epithelial-Mesenchymal Transition.

Authors - Ruijie Zhang
humanWB

Arch Med Sci

CNTN-1 promotes docetaxel resistance and epithelial-to-mesenchymal transition via the PI3K/Akt signaling pathway in prostate cancer.

Authors - Binshen Chen