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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CNTN2 Monoclonal antibody

CNTN2 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, pig, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1D3G5

Cat no : 67089-1-Ig

Synonyms

Axonal glycoprotein TAG 1, Axonin 1, AXT, CNTN2, Contactin 2, contactin 2 (axonal), DKFZp781D102, FLJ42746, TAG 1, TAG1, TAX, TAX 1, TAX1

Validation Data Gallery

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  • Western Blot (WB) analysis of pig brain tissue using CNTN2 Monoclonal antibody (67089-1-Ig)

    WB analysis of pig brain using 67089-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67089-1-Ig (CNTN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using CNTN2 Monoclonal antibody (67089-1-Ig)

    WB analysis of pig brain using 67089-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67089-1-Ig (CNTN2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig cerebellum tissue using CNTN2 Monoclonal antibody (67089-1-Ig)

    WB analysis of pig cerebellum using 67089-1-Ig

    pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 67089-1-Ig (CNTN2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig spinal cord tissue using CNTN2 Monoclonal antibody (67089-1-Ig)

    WB analysis of pig spinal cord using 67089-1-Ig

    pig spinal cord tissue were subjected to SDS PAGE followed by western blot with 67089-1-Ig (CNTN2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CNTN2 Monoclonal antibody (67089-1-Ig)

    IHC staining of mouse brain using 67089-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67089-1-Ig (CNTN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CNTN2 Monoclonal antibody (67089-1-Ig)

    IHC staining of mouse brain using 67089-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67089-1-Ig (CNTN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CNTN2 Monoclonal antibody (67089-1-Ig)

    IHC staining of mouse brain using 67089-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67089-1-Ig (CNTN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CNTN2 Monoclonal antibody (67089-1-Ig)

    IHC staining of mouse brain using 67089-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67089-1-Ig (CNTN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CNTN2 Monoclonal antibody (67089-1-Ig)

    IF Staining of mouse brain using 67089-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CNTN2 antibody (67089-1-Ig, Clone: 1D3G5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inpig brain tissue, pig cerebellum tissue, pig spinal cord tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67089-1-Ig targets CNTN2 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, pig, mouse samples.

Tested Reactivity Human, pig, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CNTN2 fusion protein Ag28301 相同性解析による交差性が予測される生物種
Full Name contactin 2 (axonal)
Calculated molecular weight 113 kDa
Observed molecular weight113-135 kDa
GenBank accession numberNM_005076
Gene symbol CNTN2
Gene ID (NCBI) 6900
RRIDAB_2918481
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CNTN2 antibody 67089-1-IgDownload protocol
IHC protocol for CNTN2 antibody 67089-1-IgDownload protocol
IF protocol for CNTN2 antibody 67089-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols