CNTROB Polyclonal antibody

CNTROB Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26880-1-AP

Synonyms

Centrobin, CNTROB, LIP8, LYST interacting protein 8, PP1221


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Tested Applications

Positive WB detected inmouse brain tissue
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26880-1-AP is CNTROB Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CNTROB fusion protein Ag25454 相同性解析による交差性が予測される生物種
Full Name centrobin, centrosomal BRCA2 interacting protein
Calculated molecular weight 903 aa, 101 kDa
Observed molecular weight 60-70 kDa, 100-130 kDa
GenBank accession numberBC021134
Gene symbol CNTROB
Gene ID (NCBI) 116840
RRIDAB_2880667
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CNTROB antibody 26880-1-APDownload protocol
IF protocol for CNTROB antibody 26880-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB,IF,

J Cell Biol

The central scaffold protein CEP350 coordinates centriole length, stability, and maturation

Authors - Onur Rojhat Karasu
humanIF

Elife

Global cellular response to chemical perturbation of PLK4 activity and abnormal centrosome number.

Authors - Johnny M Tkach
humanIF

Mol Biol Cell

The SON RNA splicing factor is required for intracellular trafficking structures that promote centriole assembly and ciliogenesis.

Authors - Alexander J Stemm-Wolf
humanIF

Science

The mechanism of acentrosomal spindle assembly in human oocytes

Authors - Tianyu Wu