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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

COA1 Polyclonal antibody

COA1 Polyclonal Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25197-1-AP

Synonyms

C7orf44, MITRAC15, Mitochondrial translation regulation assembly intermediate of cytochrome c oxidase protein of 15 kDa, Cytochrome c oxidase assembly factor 1 homolog

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using COA1 Polyclonal antibody (25197-1-AP)

    IF Staining of HeLa using 25197-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using COA1 antibody (25197-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25197-1-AP targets COA1 in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COA1 fusion protein Ag18313 相同性解析による交差性が予測される生物種
Full Name Cytochrome c oxidase assembly factor 1 homolog
Calculated molecular weight 146 aa, 17 kDa
GenBank accession numberBC056884
Gene symbol COA1
Gene ID (NCBI) 55744
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COA1, also named as C7orf44 and MITRAC15, is a mitochondrial translation regulation assembly intermediate of cytochrome c oxidase protein with MW 15 kDa. It's a component of some MITRAC complex, a cytochrome c oxidase (COX) assembly intermediate complex that regulates COX assembly. COA1 is required for assembly of mitochondrial respiratory chain complex I and complex IV.

Protocols

Product Specific Protocols
IF protocol for COA1 antibody 25197-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols