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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

COASY Polyclonal antibody

COASY Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 12991-1-AP

Synonyms

DPCK, Dephosphocoenzyme A kinase, Dephospho-CoA pyrophosphorylase, Dephospho-CoA kinase, Coenzyme A synthase

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using COASY Polyclonal antibody (12991-1-AP)

    WB analysis of HeLa using 12991-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12991-1-AP (COASY antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using COASY Polyclonal antibody (12991-1-AP)

    WB analysis of HEK-293 using 12991-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12991-1-AP (COASY antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using COASY Polyclonal antibody (12991-1-AP)

    IP experiment of HeLa using 12991-1-AP

    IP result of anti-COASY (IP:12991-1-AP, 4ug; Detection:12991-1-AP 1:500) with HeLa cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using COASY Polyclonal antibody (12991-1-AP)

    IHC staining of human colon cancer using 12991-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 12991-1-AP (COASY antibody) at dilution of 1:100 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using COASY Polyclonal antibody (12991-1-AP)

    IF Staining of HeLa using 12991-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 12991-1-AP (COASY antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12991-1-AP targets COASY in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COASY fusion protein Ag3651 相同性解析による交差性が予測される生物種
Full Name Coenzyme A synthase
Calculated molecular weight 564 aa, 62 kDa
Observed molecular weight 62 kDa
GenBank accession numberBC020985
Gene symbol COASY
Gene ID (NCBI) 80347
RRIDAB_2083528
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Bifunctional coenzyme A synthase (COASY) is a bifunctional enzyme that catalyzes the fourth and fifth sequential steps of the CoA biosynthetic pathway. The fourth reaction is catalyzed by the phosphopantetheine adenylyltransferase, coded by the coaD domain; the fifth reaction is catalyzed by the dephospho-CoA kinase, coded by the coaE domain. May act as a point of CoA biosynthesis regulation. It was reported that having a key role in erythroid maturation and identified upstream and downstream metabolites of COASY as a potential treatment for anemia in patients with MDS-RS (PMID: 36857430). COASY has 2 isoforms with molecular weights of 62 and 65 kDa.

Protocols

Product Specific Protocols
WB protocol for COASY antibody 12991-1-APDownload protocol
IHC protocol for COASY antibody 12991-1-APDownload protocol
IF protocol for COASY antibody 12991-1-APDownload protocol
IP protocol for COASY antibody 12991-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Front Pharmacol

Integrated Metabolomics and Network Pharmacology Approach to Explain Possible Action Mechanisms of Xin-Sheng-Hua Granule for Treating Anemia.

Authors - Han-Qing Pang
View Article