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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COBLL1 Polyclonal antibody (31191-1-AP)

    WB analysis using 31191-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31191-1-AP (COBLL1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using COBLL1 Polyclonal antibody (31191-1-AP)

    IHC staining of human placenta using 31191-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 31191-1-AP (COBLL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using COBLL1 Polyclonal antibody (31191-1-AP)

    IHC staining of human placenta using 31191-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 31191-1-AP (COBLL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using COBLL1 Polyclonal antibody (31191-1-AP)

    IHC staining of mouse kidney using 31191-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 31191-1-AP (COBLL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HaCaT cells using COBLL1 Polyclonal antibody (31191-1-AP)

    IF Staining of HaCaT using 31191-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HaCaT cells using COBLL1 antibody (31191-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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COBLL1 Polyclonal antibody

COBLL1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 31191-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

KIAA0977, Cordon bleu protein like 1, COBLR1, COBL like 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells
Positive IHC detected inmouse kidney tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHaCaT cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31191-1-AP targets COBLL1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COBLL1 fusion protein Ag34853 相同性解析による交差性が予測される生物種
Full Name COBL-like 1
Observed molecular weight160 kDa
GenBank accession numberBC071588
Gene Symbol COBLL1
Gene ID (NCBI) 22837
RRIDAB_3669891
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ53SF7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cordon-blue protein-like 1 (COBLL1), a negative regulator of apoptosis, is associated with lower insulin resistance. COBLL1 is involved in the cancer cell morphogenesis to a neuron-like cell shape observed in the CRPC model cells, promoting cell growth and migration (PMID: 29686105, 29686105). COBLL1 is highly expressed in most human tissues including lung, liver, kidney, pancreas, ovary, spinal cord and brain.

Protocols

Product Specific Protocols
WB protocol for COBLL1 antibody 31191-1-APDownload protocol
IHC protocol for COBLL1 antibody 31191-1-APDownload protocol
IF protocol for COBLL1 antibody 31191-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols