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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using COG1 Monoclonal antibody (68642-1-Ig)

    WB analysis using 68642-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68642-1-Ig (COG1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of LNCaP cells using COG1 Monoclonal antibody (68642-1-Ig)

    WB analysis of LNCaP using 68642-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68642-1-Ig (COG1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using COG1 Monoclonal antibody (68642-1-Ig)

    IF Staining of U2OS using 68642-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using COG1 antibody (68642-1-Ig, Clone: 2D8D11 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using COG1 Monoclonal antibody (68642-1-Ig)

    IF Staining of U2OS using 68642-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using COG1 antibody (68642-1-Ig, Clone: 2D8D11 ) at dilution of 1:1500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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COG1 Monoclonal antibody

COG1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68642-1-Ig
Clone No.2D8D11

Host / Isotype

Mouse / IgG1

Reactivity

human, rabbit

Applications

WB, IF/ICC, ELISA

Synonyms

LDLB, KIAA1381, COG complex subunit 1, CDG2G, 2D8D11

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, LNCaP cells, Jurkat cells
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68642-1-Ig targets COG1 in WB, IF/ICC, ELISA applications and shows reactivity with human, rabbit samples.

Tested Reactivity human, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen COG1 fusion protein Ag33924 相同性解析による交差性が予測される生物種
Full Name component of oligomeric golgi complex 1
Calculated molecular weight 109 kDa
Observed molecular weight100-109 kDa
GenBank accession numberBC021985
Gene Symbol COG1
Gene ID (NCBI) 9382
RRIDAB_3670386
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8WTW3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COG1 is one protein of the COG complex which is a heterooctameric complex that regulates intraGolgi trafficking and the integrity of the Golgi compartment in eukaryotic cells. It is reported that this protein is required for steps in the normal medial and trans-Golgi-associated processing of glycoconjugates and plays a role in the organization of the Golgi-localized complex. COG1 has a calculated molecular weight of 109 kDa and western blot analysis detected COG1 at 100-109 kDa (PMID: 16537452).

Protocols

Product Specific Protocols
WB protocol for COG1 antibody 68642-1-IgDownload protocol
IF protocol for COG1 antibody 68642-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols