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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

COG8 Polyclonal antibody

COG8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12661-1-AP

Synonyms

DOR1, COG complex subunit 8

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using COG8 Polyclonal antibody (12661-1-AP)

    WB analysis using 12661-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12661-1-AP (COG8 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using COG8 Polyclonal antibody (12661-1-AP)

    IHC staining of human ovary cancer using 12661-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 12661-1-AP (COG8 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using COG8 Polyclonal antibody (12661-1-AP)

    IF Staining of A431 using 12661-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using COG8 antibody (12661-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells, Raji cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12661-1-AP targets COG8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COG8 fusion protein Ag3341 相同性解析による交差性が予測される生物種
Full Name component of oligomeric golgi complex 8
Calculated molecular weight 612 aa, 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC017492
Gene symbol COG8
Gene ID (NCBI) 84342
RRIDAB_2244993
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The conserved oligomeric Golgi (COG) complex is an eight subunit (COG1-8) peripheral Golgi membrane hetero-oligomeric protein complex. The COG complex is organized into lobes A (COG2-4) and B (COG5-7) with COG1 and COG8 bridging these lobes and is thought to play a critical role in vesicle tethering processes (PMID: 17331980). Cog8 localizes exclusively in the Golgi apparatus and is involved in the retrograde Golgi transport of resident proteins responsible for sugar chain (glycan) biosynthesis (PMID: 17220172).

Protocols

Product Specific Protocols
WB protocol for COG8 antibody 12661-1-APDownload protocol
IHC protocol for COG8 antibody 12661-1-APDownload protocol
IF protocol for COG8 antibody 12661-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Commun Biol

Tankyrase-1-mediated degradation of Golgin45 regulates glycosyltransferase trafficking and protein glycosylation in Rab2-GTP-dependent manner.

Authors - Xihua Yue
View Article