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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using COIL Monoclonal antibody (67218-1-Ig)

    WB analysis of HeLa using 67218-1-Ig (same clone as 67218-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67218-1-Ig (COIL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67218-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using COIL Monoclonal antibody (67218-1-Ig)

    WB analysis of HEK-293 using 67218-1-Ig (same clone as 67218-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67218-1-Ig (COIL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67218-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using COIL Monoclonal antibody (67218-1-Ig)

    WB analysis of HepG2 using 67218-1-Ig (same clone as 67218-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67218-1-Ig (COIL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67218-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using COIL Monoclonal antibody (67218-1-Ig)

    WB analysis of Jurkat using 67218-1-Ig (same clone as 67218-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67218-1-Ig (COIL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67218-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using COIL Monoclonal antibody (67218-1-Ig)

    IF Staining of HepG2 using 67218-1-Ig (same clone as 67218-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using COIL antibody (67218-1-Ig, Clone: 2G12B8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67218-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using COIL Monoclonal antibody (67218-1-Ig)

    IF Staining of HepG2 using 67218-1-Ig (same clone as 67218-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using COIL antibody (67218-1-Ig, Clone: 2G12B8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67218-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using COIL Monoclonal antibody (67218-1-Ig)

    FC experiment of HeLa using 67218-1-Ig (same clone as 67218-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human COIL (67218-1-Ig, Clone:2G12B8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67218-1-PBS in a different storage buffer formulation.

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COIL Monoclonal antibody, PBS Only

COIL Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 67218-1-PBS
Clone No.2G12B8

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

Coilin, p80-coilin, p80 coilin, CLN80, 2G12B8

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67218-1-PBS targets COIL in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen COIL fusion protein Ag17171 相同性解析による交差性が予測される生物種
Full Name coilin
Calculated molecular weight 576 aa, 63 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC010385
Gene Symbol COIL
Gene ID (NCBI) 8161
RRIDAB_2882509
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP38432
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.