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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

COL10A1 Polyclonal antibody

COL10A1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26984-1-AP

Synonyms

COL10A1, Collagen alpha 1(X) chain, collagen, type X, alpha 1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using COL10A1 Polyclonal antibody (26984-1-AP)

    WB analysis using 26984-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26984-1-AP (COL10A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of ROS1728 cells using COL10A1 Polyclonal antibody (26984-1-AP)

    WB analysis of ROS1728 using 26984-1-AP

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 26984-1-AP (COL10A1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using COL10A1 Polyclonal antibody (26984-1-AP)

    IHC staining of mouse liver using 26984-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 26984-1-AP (COL10A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inBxPC-3 cells, ROS1728 cells, HCT 116 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

26984-1-AP targets COL10A1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COL10A1 fusion protein Ag25741 相同性解析による交差性が予測される生物種
Full Name collagen, type X, alpha 1
Calculated molecular weight 680 aa, 66 kDa
Observed molecular weight60-66 kDa
GenBank accession numberBC130621
Gene symbol COL10A1
Gene ID (NCBI) 1300
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Collagen type X alpha 1 (COL10A1), a secreted, short-chain collagen, belongs to the collagen family, which is a major interstitial matrix component specifically expressed by hypertrophic chondrocytes. COL10A1 expression is elevated in many solid tumor types, such as colon cancer, esophagus cancer, and breast cancer, and displays vital roles in many critical cellular processes (PMID: 30154451, 25321476).

Protocols

Product Specific Protocols
WB protocol for COL10A1 antibody 26984-1-APDownload protocol
IHC protocol for COL10A1 antibody 26984-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Orthop Surg Res

Gentiopicroside ameliorates the lipopolysaccharide-induced inflammatory response and hypertrophy in chondrocytes

Authors - Longfei Li
View Article